Thresholds for Sterol-Limited Growth of Daphnia magna : A Comparative Approach Using 10 Different Sterols

Martin-Creuzburg, Dominik; Oexle, Sarah; Wacker, Alexander

Thresholds for Sterol-Limited Growth of Daphnia magna : A Comparative Approach Using 10 Different Sterols

Dateien

Martin_0-260456.pdfGröße: 562.4 KBDownloads: 380

Datum

2014

Autor:innen

Martin-Creuzburg, Dominik

Oexle, Sarah

Wacker, Alexander

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Journal of Chemical Ecology. 2014, 40(9), pp. 1039-1050. ISSN 0098-0331. eISSN 1573-1561. Available under: doi: 10.1007/s10886-014-0486-1

Zusammenfassung

Arthropods are incapable of synthesizing sterols de novo and thus require a dietary source to cover their physiological demands. The most prominent sterol in animal tissues is cholesterol, which is an indispensable structural component of cell membranes and serves as precursor for steroid hormones. Instead of cholesterol, plants and algae contain a variety of different phytosterols. Consequently, herbivorous arthropods have to metabolize dietary phytosterols to cholesterol to meet their requirements for growth and reproduction. Here, we investigated sterol-limited growth responses of the freshwater herbivore Daphnia magna by supplementing a sterol-free diet with increasing amounts of 10 different phytosterols and comparing thresholds for sterol-limited growth. In addition, we analyzed the sterol composition of D. magna to explore sterol metabolic constraints and bioconversion capacities. We show that dietary phytosterols strongly differ in their potential to support somatic growth of D. magna. The dietary threshold concentrations obtained by supplementing the different sterols cover a wide range (3.5–34.4 μg mg C−1) and encompass the one for cholesterol (8.9 μg mg C−1), indicating that certain phytosterols are more efficient in supporting somatic growth than cholesterol (e.g., fucosterol, brassicasterol) while others are less efficient (e.g., dihydrocholesterol, lathosterol). The dietary sterol concentration gradients revealed that the poor quality of particular sterols can be alleviated partially by increasing dietary concentrations, and that qualitative differences among sterols are most pronounced at low to moderate dietary concentrations. We infer that the dietary sterol composition has to be considered in zooplankton nutritional ecology to accurately assess potential sterol limitations under field conditions.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Cholesterol, Daphnia, Food quality, Nutrition, Phytosterols, Sterols.

Zitieren

ISO 690

MARTIN-CREUZBURG, Dominik, Sarah OEXLE, Alexander WACKER, 2014. Thresholds for Sterol-Limited Growth of Daphnia magna : A Comparative Approach Using 10 Different Sterols. In: Journal of Chemical Ecology. 2014, 40(9), pp. 1039-1050. ISSN 0098-0331. eISSN 1573-1561. Available under: doi: 10.1007/s10886-014-0486-1

BibTex

@article{MartinCreuzburg2014Thres-29656,
  year={2014},
  doi={10.1007/s10886-014-0486-1},
  title={Thresholds for Sterol-Limited Growth of Daphnia magna : A Comparative Approach Using 10 Different Sterols},
  number={9},
  volume={40},
  issn={0098-0331},
  journal={Journal of Chemical Ecology},
  pages={1039--1050},
  author={Martin-Creuzburg, Dominik and Oexle, Sarah and Wacker, Alexander}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29656">
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/29656/3/Martin_0-260456.pdf"/>
    <dc:language>eng</dc:language>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/29656/3/Martin_0-260456.pdf"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/29656"/>
    <dcterms:issued>2014</dcterms:issued>
    <dc:contributor>Martin-Creuzburg, Dominik</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2015-01-30T08:01:43Z</dcterms:available>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Martin-Creuzburg, Dominik</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Wacker, Alexander</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Oexle, Sarah</dc:creator>
    <dc:rights>terms-of-use</dc:rights>
    <dc:contributor>Oexle, Sarah</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2015-01-30T08:01:43Z</dc:date>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dcterms:abstract xml:lang="eng">Arthropods are incapable of synthesizing sterols de novo and thus require a dietary source to cover their physiological demands. The most prominent sterol in animal tissues is cholesterol, which is an indispensable structural component of cell membranes and serves as precursor for steroid hormones. Instead of cholesterol, plants and algae contain a variety of different phytosterols. Consequently, herbivorous arthropods have to metabolize dietary phytosterols to cholesterol to meet their requirements for growth and reproduction. Here, we investigated sterol-limited growth responses of the freshwater herbivore Daphnia magna by supplementing a sterol-free diet with increasing amounts of 10 different phytosterols and comparing thresholds for sterol-limited growth. In addition, we analyzed the sterol composition of D. magna to explore sterol metabolic constraints and bioconversion capacities. We show that dietary phytosterols strongly differ in their potential to support somatic growth of D. magna. The dietary threshold concentrations obtained by supplementing the different sterols cover a wide range (3.5–34.4 μg mg C−1) and encompass the one for cholesterol (8.9 μg mg C−1), indicating that certain phytosterols are more efficient in supporting somatic growth than cholesterol (e.g., fucosterol, brassicasterol) while others are less efficient (e.g., dihydrocholesterol, lathosterol). The dietary sterol concentration gradients revealed that the poor quality of particular sterols can be alleviated partially by increasing dietary concentrations, and that qualitative differences among sterols are most pronounced at low to moderate dietary concentrations. We infer that the dietary sterol composition has to be considered in zooplankton nutritional ecology to accurately assess potential sterol limitations under field conditions.</dcterms:abstract>
    <dcterms:title>Thresholds for Sterol-Limited Growth of Daphnia magna : A Comparative Approach Using 10 Different Sterols</dcterms:title>
    <dc:creator>Wacker, Alexander</dc:creator>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Ja