The Effect of Cyanobacterial Biomass Enrichment by Centrifugation and GF/C Filtration on Subsequent Microcystin Measurement

Rogers, Shelley; Puddick, Jonathan; Wood, Susanna A.; Dietrich, Daniel R.; Hamilton, David P.; Prinsep, Michele R.

The Effect of Cyanobacterial Biomass Enrichment by Centrifugation and GF/C Filtration on Subsequent Microcystin Measurement

Dateien

Rogers_0-291150.pdfGröße: 696.88 KBDownloads: 281

Datum

2015

Autor:innen

Rogers, Shelley

Puddick, Jonathan

Wood, Susanna A.

Dietrich, Daniel R.

Hamilton, David P.

Prinsep, Michele R.

Open Access-Veröffentlichung

Open Access Gold

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Toxins. 2015, 7(3), pp. 821-834. eISSN 2072-6651. Available under: doi: 10.3390/toxins7030821

Zusammenfassung

Microcystins are cyclic peptides produced by multiple cyanobacterial genera. After accumulation in the liver of animals they inhibit eukaryotic serine/threonine protein phosphatases, causing liver disease or death. Accurate detection/quantification of microcystins is essential to ensure safe water resources and to enable research on this toxin. Previous methodological comparisons have focused on detection and extraction techniques, but have not investigated the commonly used biomass enrichment steps. These enrichment steps could modulate toxin production as recent studies have demonstrated that high cyanobacterial cell densities cause increased microcystin levels. In this study, three microcystin-producing strains were processed using no cell enrichment steps (by direct freezing at three temperatures) and with biomass enrichment (by centrifugation or GF/C filtration). After extraction, microcystins were analyzed using liquid chromatography-tandem mass spectrometry. All processing methods tested, except GF/C filtration, resulted in comparable microcystin quotas for all strains. The low yields observed for the filtration samples were caused by adsorption of arginine-containing microcystins to the GF/C filters. Whilst biomass enrichment did not affect microcystin metabolism over the time-frame of normal sample processing, problems associated with GF/C filtration were identified. The most widely applicable processing method was direct freezing of samples as it could be utilized in both field and laboratory environments.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

microcystin; cyanobacteria; microcystis; planktothrix; biomass concentration; sample processing

Zitieren

ISO 690

ROGERS, Shelley, Jonathan PUDDICK, Susanna A. WOOD, Daniel R. DIETRICH, David P. HAMILTON, Michele R. PRINSEP, 2015. The Effect of Cyanobacterial Biomass Enrichment by Centrifugation and GF/C Filtration on Subsequent Microcystin Measurement. In: Toxins. 2015, 7(3), pp. 821-834. eISSN 2072-6651. Available under: doi: 10.3390/toxins7030821

BibTex

@article{Rogers2015Effec-31512,
  year={2015},
  doi={10.3390/toxins7030821},
  title={The Effect of Cyanobacterial Biomass Enrichment by Centrifugation and GF/C Filtration on Subsequent Microcystin Measurement},
  number={3},
  volume={7},
  journal={Toxins},
  pages={821--834},
  author={Rogers, Shelley and Puddick, Jonathan and Wood, Susanna A. and Dietrich, Daniel R. and Hamilton, David P. and Prinsep, Michele R.}
}

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