@article{Wu2015Analy-31535,
  year={2015},
  doi={10.1016/j.jim.2015.02.008},
  title={Analysis of CCR7 mediated T cell transfectant migration using a microfluidic gradient generator},
  volume={419},
  issn={0022-1759},
  journal={Journal of Immunological Methods},
  pages={9--17},
  author={Wu, Xun and Wu, Jiandong and Li, Hongzhao and Legler, Daniel F. and Marshall, Aaron J. and Lin, Francis}
}

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    <dc:contributor>Wu, Xun</dc:contributor>
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    <dc:contributor>Wu, Jiandong</dc:contributor>
    <dcterms:abstract xml:lang="eng">T lymphocyte migration is crucial for adaptive immunity. Manipulation of signaling molecules controlling cell migration combined with in-vitro cell migration analysis provides a powerful research approach. Microfluidic devices, which can precisely configure chemoattractant gradients and allow quantitative single cell analysis, have been increasingly applied to cell migration and chemotaxis studies. However, there are a very limited number of published studies involving microfluidic migration analysis of genetically manipulated immune cells. In this study, we describe a simple microfluidic method for quantitative analysis of T cells expressing transfected chemokine receptors and other cell migration signaling probes. Using this method, we demonstrated chemotaxis of Jurkat transfectants expressing wild-type or C-terminus mutated CCR7 within a gradient of chemokine CCL19, and characterized the difference in transfectant migration mediated by wild-type and mutant CCR7. The EGFP-tagged CCR7 allows identification of CCR7-expressing transfectants in cell migration analysis and microscopy assessment of CCR7 dynamics. Collectively, our study demonstrated the effective use of the microfluidic method for studying CCR7 mediated T cell transfectant migration. We envision this developed method will provide a useful platform to functionally test various signaling mechanisms at the cell migration level.</dcterms:abstract>
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    <dc:contributor>Marshall, Aaron J.</dc:contributor>
    <dc:creator>Lin, Francis</dc:creator>
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    <dc:creator>Wu, Xun</dc:creator>
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    <dc:contributor>Lin, Francis</dc:contributor>
    <dc:creator>Li, Hongzhao</dc:creator>
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    <dc:creator>Legler, Daniel F.</dc:creator>
    <dcterms:title>Analysis of CCR7 mediated T cell transfectant migration using a microfluidic gradient generator</dcterms:title>
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    <dc:contributor>Li, Hongzhao</dc:contributor>
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    <dc:contributor>Legler, Daniel F.</dc:contributor>
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