What causes mating system shifts in plants? : Arabidopsis lyrata as a case study

Mable, Barbara K.; Hagmann, J.; Kim, S.-T.; Adam, Aileen; Kilbride, Elizabeth; Weigel, Detlef; Stift, Marc

What causes mating system shifts in plants? : Arabidopsis lyrata as a case study

Dateien

Mable_0-373353.pdfGröße: 1.37 MBDownloads: 311

Datum

2017

Autor:innen

Mable, Barbara K.

Hagmann, J.

Kim, S.-T.

Adam, Aileen

Kilbride, Elizabeth

Weigel, Detlef

Stift, Marc

Open Access-Veröffentlichung

Open Access Hybrid

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Heredity. 2017, 118(1), pp. 52-63. ISSN 0018-067X. eISSN 1365-2540. Available under: doi: 10.1038/hdy.2016.99

Zusammenfassung

The genetic breakdown of self-incompatibility (SI) and subsequent mating system shifts to inbreeding has intrigued evolutionary geneticists for decades. Most of our knowledge is derived from interspecific comparisons between inbreeding species and their outcrossing relatives, where inferences may be confounded by secondary mutations that arose after the initial loss of SI. Here, we study an intraspecific breakdown of SI and its consequences in North American Arabidopsis lyrata to test whether: (1) particular S-locus haplotypes are associated with the loss of SI and/or the shift to inbreeding; (2) a population bottleneck may have played a role in driving the transition to inbreeding; and (3) the mutation(s) underlying the loss of SI are likely to have occurred at the S-locus. Combining multiple approaches for genotyping, we found that outcrossing populations on average harbour 5 to 9 S-locus receptor kinase (SRK) alleles, but only two, S1 and S19, are shared by most inbreeding populations. Self-compatibility (SC) behaved genetically as a recessive trait, as expected from a loss-of-function mutation. Bulked segregant analysis in SC × SI F2 individuals using deep sequencing confirmed that all SC plants were S1 homozygotes but not all S1 homozygotes were SC. This was also revealed in population surveys, where only a few S1 homozygotes were SC. Together with crossing data, this suggests that there is a recessive factor that causes SC that is physically unlinked to the S-locus. Overall, our results emphasise the value of combining classical genetics with advanced sequencing approaches to resolve long outstanding questions in evolutionary biology.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Self-compatibility, self-incompatibility , inbreeding

Zitieren

ISO 690

MABLE, Barbara K., J. HAGMANN, S.-T. KIM, Aileen ADAM, Elizabeth KILBRIDE, Detlef WEIGEL, Marc STIFT, 2017. What causes mating system shifts in plants? : Arabidopsis lyrata as a case study. In: Heredity. 2017, 118(1), pp. 52-63. ISSN 0018-067X. eISSN 1365-2540. Available under: doi: 10.1038/hdy.2016.99

BibTex

@article{Mable2017-01cause-36059,
  year={2017},
  doi={10.1038/hdy.2016.99},
  title={What causes mating system shifts in plants? : Arabidopsis lyrata as a case study},
  number={1},
  volume={118},
  issn={0018-067X},
  journal={Heredity},
  pages={52--63},
  author={Mable, Barbara K. and Hagmann, J. and Kim, S.-T. and Adam, Aileen and Kilbride, Elizabeth and Weigel, Detlef and Stift, Marc}
}

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