Single-particle evanescent light scattering simulations for total internal reflection microscopy

Helden, Laurent; Eremina, Elena; Riefler, Norbert; Hertlein, Christopher; Bechinger, Clemens; Eremin, Yuri; Wriedt, Thomas

Single-particle evanescent light scattering simulations for total internal reflection microscopy

Dateien

Helden_0-410596.pdfGröße: 151.77 KBDownloads: 338

Datum

2006

Autor:innen

Helden, Laurent

Eremina, Elena

Riefler, Norbert

Hertlein, Christopher

Bechinger, Clemens

Eremin, Yuri

Wriedt, Thomas

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Physik

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Applied Optics. 2006, 45(28), pp. 7299-7308. ISSN 0003-6935. eISSN 1539-4522. Available under: doi: 10.1364/AO.45.007299

Zusammenfassung

We simulate and measure light scattering of a micrometer-sized spherical particle suspended in solution close to a glass substrate. The model, based on the discrete sources method, is developed to describe the experimental situation of total internal reflection microscopy experiments; i.e., the particle is illuminated by an evanescent light field originating from the glass–solvent interface. In contrast to the well-established assumption of a simple exponential decay of the scattering intensity with distance, we demonstrate significant deviations for a certain range of penetration depths and polarization states of the incident light.

Fachgebiet (DDC)

530 Physik

Zitieren

ISO 690

HELDEN, Laurent, Elena EREMINA, Norbert RIEFLER, Christopher HERTLEIN, Clemens BECHINGER, Yuri EREMIN, Thomas WRIEDT, 2006. Single-particle evanescent light scattering simulations for total internal reflection microscopy. In: Applied Optics. 2006, 45(28), pp. 7299-7308. ISSN 0003-6935. eISSN 1539-4522. Available under: doi: 10.1364/AO.45.007299

BibTex

@article{Helden2006-10-01Singl-39212,
  year={2006},
  doi={10.1364/AO.45.007299},
  title={Single-particle evanescent light scattering simulations for total internal reflection microscopy},
  number={28},
  volume={45},
  issn={0003-6935},
  journal={Applied Optics},
  pages={7299--7308},
  author={Helden, Laurent and Eremina, Elena and Riefler, Norbert and Hertlein, Christopher and Bechinger, Clemens and Eremin, Yuri and Wriedt, Thomas}
}

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