USE1 is a bispecific conjugating enzyme for ubiquitin and FAT10, which FAT10ylates itself in cis

Aichem, Annette; Pelzer, Christiane; Lukasiak, Sebastian; Kalveram, Birte; Sheppard, Paul W.; Rani, Neha; Schmidtke, Gunter; Gröttrup, Marcus

USE1 is a bispecific conjugating enzyme for ubiquitin and FAT10, which FAT10ylates itself in cis

Dateien

Aichem_125773.pdfGröße: 1.59 MBDownloads: 225

Datum

2010

Autor:innen

Aichem, Annette

Pelzer, Christiane

Lukasiak, Sebastian

Kalveram, Birte

Sheppard, Paul W.

Rani, Neha

Schmidtke, Gunter

Gröttrup, Marcus

Open Access-Veröffentlichung

Open Access Gold

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Nature Communications. 2010, 1(2), pp. 1-10. eISSN 2041-1723. Available under: doi: 10.1038/ncomms1012

Zusammenfassung

The ubiquitin-like modifier FAT10 targets proteins for degradation by the proteasome and is activated by the E1 enzyme UBA6. In this study, we identify the UBA6-specific E2 enzyme (USE1) as an interaction partner of FAT10. Activated FAT10 can be transferred from UBA6 onto USE1 in vitro, and endogenous USE1 and FAT10 can be coimmunoprecipitated from intact cells. Small interfering RNA-mediated downregulation of USE1 mRNA resulted in a strong reduction of FAT10 conjugate formation under endogenous conditions, suggesting that USE1 is a major E2 enzyme in the FAT10 conjugation cascade. Interestingly, USE1 is not only the first E2 enzyme but also the first known substrate of FAT10 conjugation, as it was efficiently auto-FAT10ylated in cis but not in trans.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

AICHEM, Annette, Christiane PELZER, Sebastian LUKASIAK, Birte KALVERAM, Paul W. SHEPPARD, Neha RANI, Gunter SCHMIDTKE, Marcus GRÖTTRUP, 2010. USE1 is a bispecific conjugating enzyme for ubiquitin and FAT10, which FAT10ylates itself in cis. In: Nature Communications. 2010, 1(2), pp. 1-10. eISSN 2041-1723. Available under: doi: 10.1038/ncomms1012

BibTex

@article{Aichem2010bispe-12577,
  year={2010},
  doi={10.1038/ncomms1012},
  title={USE1 is a bispecific conjugating enzyme for ubiquitin and FAT10, which FAT10ylates itself in cis},
  number={2},
  volume={1},
  journal={Nature Communications},
  pages={1--10},
  author={Aichem, Annette and Pelzer, Christiane and Lukasiak, Sebastian and Kalveram, Birte and Sheppard, Paul W. and Rani, Neha and Schmidtke, Gunter and Gröttrup, Marcus}
}

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