@article{Luckschander2009-11Pheno-14258,
  year={2009},
  doi={10.1051/vetres/2009042},
  title={Phenotyping, functional characterization, and developmental changes in canine intestinal intraepithelial lymphocytes},
  number={6},
  volume={40},
  issn={0928-4249},
  journal={Veterinary Research},
  pages={58--11},
  author={Luckschander, Nicole and Pfammatter, Nadia S. and Sidler, Daniel and Jakob, Sabine and Burgener, Iwan A. and Moore, Peter F. and Zurbriggen, Andreas and Corazza, Nadia and Brunner, Thomas}
}

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/14258">
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-08-02T07:18:11Z</dcterms:available>
    <dc:contributor>Pfammatter, Nadia S.</dc:contributor>
    <dc:creator>Zurbriggen, Andreas</dc:creator>
    <dc:creator>Sidler, Daniel</dc:creator>
    <dc:creator>Burgener, Iwan A.</dc:creator>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/14258/2/Luckschander_142586.pdf"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-08-02T07:18:11Z</dc:date>
    <dc:creator>Brunner, Thomas</dc:creator>
    <dc:creator>Corazza, Nadia</dc:creator>
    <dc:contributor>Luckschander, Nicole</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Moore, Peter F.</dc:contributor>
    <dc:contributor>Brunner, Thomas</dc:contributor>
    <dcterms:issued>2009-11</dcterms:issued>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/14258"/>
    <dc:contributor>Sidler, Daniel</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:contributor>Zurbriggen, Andreas</dc:contributor>
    <dc:contributor>Burgener, Iwan A.</dc:contributor>
    <dc:creator>Moore, Peter F.</dc:creator>
    <dcterms:bibliographicCitation>First publ. in: Veterinary Research 40 (2009), 6, pp. 1-11</dcterms:bibliographicCitation>
    <dc:language>eng</dc:language>
    <dc:creator>Pfammatter, Nadia S.</dc:creator>
    <dcterms:abstract>Little is currently known about the lymphocyte populations in the normal and diseased canine gut. The aim of this study was thus the phenotypical and functional characterization of canine intestinal intraepithelial lymphocytes (IEL). IEL were isolated from full-thickness biopsies of 15 adult Swiss Beagle dogs (mean age 8.2 +/-2.8 years) and compared to mesenteric lymph node cells. The phenotypical characterization by multi-parameter flow cytometry revealed that canine IEL differ substantially from lymph node T cells, and consist of various unconventional lymphocyte subsets, unique to mucosal surfaces. These include gammasigma T cells, and CD4(-)CD8(-) and CD8alphaalpha(+) T cells. IEL populations in adult dogs were also compared to those isolated from neonatal Beagle dogs. Analysis revealed a high frequency of undifferentiated CD4(-)CD8(-) T cells in newborn dogs whereas mature CD4(+) and CD8(+) T cells predominate in adult dogs, indicating maturation of the intestinal immune system during development. As IEL in other species are thought to exhibit regulatory functions, we investigated the role of IEL on the activation-induced proliferation of lymph node T cells. While IEL alone did not show activation-induced proliferation, they significantly inhibited the proliferation of activated lymph node T cells in a cell number-dependent manner. These findings are the first to demonstrate that canine intestinal IEL have an immunoregulatory phenotype, which may contribute to the maintenance of intestinal immune homeostasis and may, therefore, be lost in canine chronic enteropathies.</dcterms:abstract>
    <dc:contributor>Jakob, Sabine</dc:contributor>
    <dc:contributor>Corazza, Nadia</dc:contributor>
    <dcterms:title>Phenotyping, functional characterization, and developmental changes in canine intestinal intraepithelial lymphocytes</dcterms:title>
    <dc:rights>terms-of-use</dc:rights>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/14258/2/Luckschander_142586.pdf"/>
    <dc:creator>Jakob, Sabine</dc:creator>
    <dc:creator>Luckschander, Nicole</dc:creator>
  </rdf:Description>
</rdf:RDF>