Distinct requirements for activation-induced cell surface expression of preformed Fas/CD95 ligand and cytolytic granule markers in T cells

Kassahn, Daniela; Nachbur, Ueli; Conus, Sébastien; Micheau, Olivier; Schneider, Pascal; Simon, Hans-Uwe; Brunner, Thomas

Distinct requirements for activation-induced cell surface expression of preformed Fas/CD95 ligand and cytolytic granule markers in T cells

Dateien

2009_Kassahn_Distinct requirements.pdfGröße: 336.61 KBDownloads: 308

Datum

2009

Autor:innen

Kassahn, Daniela

Nachbur, Ueli

Conus, Sébastien

Micheau, Olivier

Schneider, Pascal

Simon, Hans-Uwe

Brunner, Thomas

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Cell Death and Differentiation. 2009, 16(1), pp. 115-124. ISSN 1350-9047. eISSN 1476-5403. Available under: doi: 10.1038/cdd.2008.133

Zusammenfassung

Fas (CD95/Apo-1) ligand is a potent inducer of apoptosis and one of the major killing effector mechanisms of cytotoxic T cells. Thus, Fas ligand activity has to be tightly regulated, involving various transcriptional and post-transcriptional processes. For example, preformed Fas ligand is stored in secretory lysosomes of activated T cells, and rapidly released by degranulation upon reactivation. In this study, we analyzed the minimal requirements for activation-induced degranulation of Fas ligand. T cell receptor activation can be mimicked by calcium ionophore and phorbol ester. Unexpectedly, we found that stimulation with phorbol ester alone is sufficient to trigger Fas ligand release, whereas calcium ionophore is neither sufficient nor necessary. The relevance of this process was confirmed in primary CD4+ and CD8+ T cells and NK cells. Although the activation of protein kinase(s) was absolutely required for Fas ligand degranulation, protein kinase C or A were not involved. Previous reports have shown that preformed Fas ligand co-localizes with other markers of cytolytic granules. We found, however, that the activation-induced degranulation of Fas ligand has distinct requirements and involves different mechanisms than those of the granule markers CD63 and CD107a/Lamp-1. We conclude that activation-induced degranulation of Fas ligand in cytotoxic lymphocytes is differently regulated than other classical cytotoxic granule proteins.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

cytotoxic T cells, Fas ligand, cytotoxic granules, degranulation, protein kinases

Zitieren

ISO 690

KASSAHN, Daniela, Ueli NACHBUR, Sébastien CONUS, Olivier MICHEAU, Pascal SCHNEIDER, Hans-Uwe SIMON, Thomas BRUNNER, 2009. Distinct requirements for activation-induced cell surface expression of preformed Fas/CD95 ligand and cytolytic granule markers in T cells. In: Cell Death and Differentiation. 2009, 16(1), pp. 115-124. ISSN 1350-9047. eISSN 1476-5403. Available under: doi: 10.1038/cdd.2008.133

BibTex

@article{Kassahn2009-01Disti-14260,
  year={2009},
  doi={10.1038/cdd.2008.133},
  title={Distinct requirements for activation-induced cell surface expression of preformed Fas/CD95 ligand and cytolytic granule markers in T cells},
  number={1},
  volume={16},
  issn={1350-9047},
  journal={Cell Death and Differentiation},
  pages={115--124},
  author={Kassahn, Daniela and Nachbur, Ueli and Conus, Sébastien and Micheau, Olivier and Schneider, Pascal and Simon, Hans-Uwe and Brunner, Thomas}
}

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