@article{Brumatti2008-02-01Conve-14270,
  year={2008},
  doi={10.1016/j.yexcr.2007.11.003},
  title={Conversion of CD95 (Fas) Type II into Type I signaling by sub-lethal doses of cycloheximide},
  number={3},
  volume={314},
  issn={0014-4827},
  journal={Experimental Cell Research},
  pages={554--563},
  author={Brumatti, Gabriela and Yon, Monica and Castro, Fabiola A. and Bueno-da-Silva, Ana Elisa Barreiros and Jacysyn, Jacqueline F. and Brunner, Thomas and Amarante-Mendes, Gustavo P.}
}

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    <dc:contributor>Brunner, Thomas</dc:contributor>
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    <dc:creator>Yon, Monica</dc:creator>
    <dcterms:title>Conversion of CD95 (Fas) Type II into Type I signaling by sub-lethal doses of cycloheximide</dcterms:title>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-10-19T13:33:15Z</dc:date>
    <dc:creator>Brumatti, Gabriela</dc:creator>
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    <dc:contributor>Jacysyn, Jacqueline F.</dc:contributor>
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    <dc:contributor>Brumatti, Gabriela</dc:contributor>
    <dc:creator>Brunner, Thomas</dc:creator>
    <dc:creator>Jacysyn, Jacqueline F.</dc:creator>
    <dc:contributor>Bueno-da-Silva, Ana Elisa Barreiros</dc:contributor>
    <dc:contributor>Castro, Fabiola A.</dc:contributor>
    <dc:contributor>Amarante-Mendes, Gustavo P.</dc:contributor>
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    <dc:creator>Bueno-da-Silva, Ana Elisa Barreiros</dc:creator>
    <dcterms:abstract xml:lang="eng">CD95 (Fas/Apo-1)-mediated apoptosis was shown to occur through two distinct pathways. One involves a direct activation of caspase-3 by large amounts of caspase-8 generated at the DISC (Type I cells). The other is related to the cleavage of Bid by low concentration of caspase-8, leading to the release of cytochrome c from mitochondria and the activation of caspase-3 by the cytochrome c/APAF-1/caspase-9 apoptosome (Type II cells). It is also known that the protein synthesis inhibitor cycloheximide (CHX) sensitizes Type I cells to CD95-mediated apoptosis, but it remains contradictory whether this effect also occurs in Type II cells. Here, we show that sub-lethal doses of CHX render both Type I and Type II cells sensitive to the apoptogenic effect of anti-CD95 antibodies but not to chemotherapeutic drugs. Moreover, Bcl-2-positive Type II cells become strongly sensitive to CD95-mediated apoptosis by the addition of CHX to the cell culture. This is not the result of a restraint of the anti-apoptotic effect of Bcl-2 at the mitochondrial level since CHX-treated Type II cells still retain their resistance to chemotherapeutic drugs. Therefore, CHX treatment is granting the CD95-mediated pathway the ability to bypass the mitochondria requirement to apoptosis, much alike to what is observed in Type I cells.</dcterms:abstract>
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    <dc:creator>Amarante-Mendes, Gustavo P.</dc:creator>
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    <dc:contributor>Yon, Monica</dc:contributor>
    <dcterms:bibliographicCitation>First publ. in: Experimental Cell Research ; 314 (2008), 3. - pp. 554-563</dcterms:bibliographicCitation>
    <dcterms:issued>2008-02-01</dcterms:issued>
    <dc:creator>Castro, Fabiola A.</dc:creator>
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