Lack of evidence for a role of hydrophobins in conferring surface hydrophobicity to conidia and hyphae of Botrytis cinerea

Mosbach, Andreas; Leroch, Michaela; Mendgen, Kurt; Hahn, Matthias

Lack of evidence for a role of hydrophobins in conferring surface hydrophobicity to conidia and hyphae of Botrytis cinerea

Dateien

mosbach 2011.pdfGröße: 2.32 MBDownloads: 283

Datum

2011

Autor:innen

Mosbach, Andreas

Leroch, Michaela

Mendgen, Kurt

Hahn, Matthias

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

BMC Microbiology. 2011, 11(1), 10. eISSN 1471-2180. Available under: doi: 10.1186/1471-2180-11-10

Zusammenfassung

Background: Hydrophobins are small, cysteine rich, surface active proteins secreted by filamentous fungi, forming hydrophobic layers on the walls of aerial mycelia and spores. Hydrophobin mutants in a variety of fungi have been described to show ‘easily wettable’ phenotypes, indicating that hydrophobins play a general role in conferring surface hydrophobicity to aerial hyphae and spores.

Results: In the genome of the grey mould fungus Botrytis cinerea, genes encoding three hydrophobins and six hydrophobin-like proteins were identified. Expression analyses revealed low or no expression of these genes in conidia, while some of them showed increased or specific expression in other stages, such as sclerotia or fruiting
bodies. Bhp1 belongs to the class I hydrophobins, whereas Bhp2 and Bhp3 are members of hydrophobin class II. Single, double and triple hydrophobin knock-out mutants were constructed by consecutively deleting bhp1, bhp2 and bhp3. In addition, a mutant in the hydrophobin-like gene bhl1 was generated. The mutants were tested for
germination and growth under different conditions, formation of sclerotia, ability to penetrate and infect host tissue, and for spore and mycelium surface properties. Surprisingly, none of the B. cinerea hydrophobin mutants showed obvious phenotypic defects in any of these characters. Scanning electron microscopy of the hydrophobic
conidial surfaces did not reveal evidence for the presence of typical hydrophobin ‘rodlet’ layers.

Conclusions: These data provide evidence that in B. cinerea, hydrophobins are not involved in conferring surface hydrophobicity to conidia and aerial hyphae, and challenge their universal role in filamentous fungi. The function of some of these proteins in sclerotia and fruiting bodies remains to be investigated.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

MOSBACH, Andreas, Michaela LEROCH, Kurt MENDGEN, Matthias HAHN, 2011. Lack of evidence for a role of hydrophobins in conferring surface hydrophobicity to conidia and hyphae of Botrytis cinerea. In: BMC Microbiology. 2011, 11(1), 10. eISSN 1471-2180. Available under: doi: 10.1186/1471-2180-11-10

BibTex

@article{Mosbach2011evide-16545,
  year={2011},
  doi={10.1186/1471-2180-11-10},
  title={Lack of evidence for a role of hydrophobins in conferring surface hydrophobicity to conidia and hyphae of Botrytis cinerea},
  number={1},
  volume={11},
  journal={BMC Microbiology},
  author={Mosbach, Andreas and Leroch, Michaela and Mendgen, Kurt and Hahn, Matthias},
  note={Article Number: 10}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/16545">
    <dc:language>eng</dc:language>
    <dc:creator>Mendgen, Kurt</dc:creator>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/16545/2/mosbach%202011.pdf"/>
    <dc:contributor>Mendgen, Kurt</dc:contributor>
    <dcterms:issued>2011</dcterms:issued>
    <dc:contributor>Mosbach, Andreas</dc:contributor>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Leroch, Michaela</dc:contributor>
    <dcterms:title>Lack of evidence for a role of hydrophobins in conferring surface hydrophobicity to conidia and hyphae of Botrytis cinerea</dcterms:title>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-11-10T09:50:57Z</dc:date>
    <dc:creator>Hahn, Matthias</dc:creator>
    <dc:creator>Leroch, Michaela</dc:creator>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/16545"/>
    <dc:creator>Mosbach, Andreas</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:rights>terms-of-use</dc:rights>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Hahn, Matthias</dc:contributor>
    <dcterms:bibliographicCitation>First publ. in: BMC Microbiology (2011). - 11:10</dcterms:bibliographicCitation>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/16545/2/mosbach%202011.pdf"/>
    <dcterms:abstract xml:lang="eng">Background: Hydrophobins are small, cysteine rich, surface active proteins secreted by filamentous fungi, forming hydrophobic layers on the walls of aerial mycelia and spores. Hydrophobin mutants in a variety of fungi have been described to show ‘easily wettable’ phenotypes, indicating that hydrophobins play a general role in conferring surface hydrophobicity to aerial hyphae and spores.&lt;br /&gt;&lt;br /&gt;Results: In the genome of the grey mould fungus Botrytis cinerea, genes encoding three hydrophobins and six hydrophobin-like proteins were identified. Expression analyses revealed low or no expression of these genes in conidia, while some of them showed increased or specific expression in other stages, such as sclerotia or fruiting&lt;br /&gt;bodies. Bhp1 belongs to the class I hydrophobins, whereas Bhp2 and Bhp3 are members of hydrophobin class II. Single, double and triple hydrophobin knock-out mutants were constructed by consecutively deleting bhp1, bhp2 and bhp3. In addition, a mutant in the hydrophobin-like gene bhl1 was generated. The mutants were tested for&lt;br /&gt;germination and growth under different conditions, formation of sclerotia, ability to penetrate and infect host tissue, and for spore and mycelium surface properties. Surprisingly, none of the B. cinerea hydrophobin mutants showed obvious phenotypic defects in any of these characters. Scanning electron microscopy of the hydrophobic&lt;br /&gt;conidial surfaces did not reveal evidence for the presence of typical hydrophobin ‘rodlet’ layers.&lt;br /&gt;&lt;br /&gt;Conclusions: These data provide evidence that in B. cinerea, hydrophobins are not involved in conferring surface hydrophobicity to conidia and aerial hyphae, and challenge their universal role in filamentous fungi. The function of some of these proteins in sclerotia and fruiting bodies remains to be investigated.</dcterms:abstract>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-11-10T09:50:57Z</dcterms:available>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Ja