Gene Expression Variation Resolves Species and Individual Strains among Coral-Associated Dinoflagellates within the Genus Symbiodinium

Parkinson, John E.; Baumgarten, Sebastian; Michell, Craig T.; Baums, Iliana B; LaJeunesse, Todd C.; Voolstra, Christian R.

Gene Expression Variation Resolves Species and Individual Strains among Coral-Associated Dinoflagellates within the Genus Symbiodinium

Dateien

Parkinson_2-10d3qp5lyu063.pdfGröße: 1.1 MBDownloads: 353

Datum

2016

Autor:innen

Parkinson, John E.

Baumgarten, Sebastian

Michell, Craig T.

Baums, Iliana B

LaJeunesse, Todd C.

Voolstra, Christian R.

Open Access-Veröffentlichung

Open Access Gold

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Genome biology and evolution. Oxford University Press. 2016, 8(3), pp. 665-680. eISSN 1759-6653. Available under: doi: 10.1093/gbe/evw019

Zusammenfassung

Reef-building corals depend on symbiotic mutualisms with photosynthetic dinoflagellates in the genus Symbiodinium. This large microalgal group comprises many highly divergent lineages ("Clades A-I") and hundreds of undescribed species. Given their ecological importance, efforts have turned to genomic approaches to characterize the functional ecology of Symbiodinium. To date, investigators have only compared gene expression between representatives from separate clades-the equivalent of contrasting genera or families in other dinoflagellate groups-making it impossible to distinguish between clade-level and species-level functional differences. Here, we examined the transcriptomes of four species within one Symbiodinium clade (Clade B) at ∼20,000 orthologous genes, as well as multiple isoclonal cell lines within species (i.e., cultured strains). These species span two major adaptive radiations within Clade B, each encompassing both host-specialized and ecologically cryptic taxa. Species-specific expression differences were consistently enriched for photosynthesis-related genes, likely reflecting selection pressures driving niche diversification. Transcriptional variation among strains involved fatty acid metabolism and biosynthesis pathways. Such differences among individuals are potentially a major source of physiological variation, contributing to the functional diversity of coral holobionts composed of unique host-symbiont genotype pairings. Our findings expand the genomic resources available for this important symbiont group and emphasize the power of comparative transcriptomics as a method for studying speciation processes and interindividual variation in nonmodel organisms.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

dinoflagellates, phylogenetics, RNAseq, symbiosis, transcriptome, zooxanthellae

Zitieren

ISO 690

PARKINSON, John E., Sebastian BAUMGARTEN, Craig T. MICHELL, Iliana B BAUMS, Todd C. LAJEUNESSE, Christian R. VOOLSTRA, 2016. Gene Expression Variation Resolves Species and Individual Strains among Coral-Associated Dinoflagellates within the Genus Symbiodinium. In: Genome biology and evolution. Oxford University Press. 2016, 8(3), pp. 665-680. eISSN 1759-6653. Available under: doi: 10.1093/gbe/evw019

BibTex

@article{Parkinson2016-02-11Expre-51456,
  year={2016},
  doi={10.1093/gbe/evw019},
  title={Gene Expression Variation Resolves Species and Individual Strains among Coral-Associated Dinoflagellates within the Genus Symbiodinium},
  number={3},
  volume={8},
  journal={Genome biology and evolution},
  pages={665--680},
  author={Parkinson, John E. and Baumgarten, Sebastian and Michell, Craig T. and Baums, Iliana B and LaJeunesse, Todd C. and Voolstra, Christian R.}
}

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Universitätsbibliographie

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Begutachtet

Ja