@article{Kuhn2022-10-15Singl-59023,
  year={2022},
  doi={10.1038/s41467-022-33704-z},
  title={Single-molecule tracking of Nodal and Lefty in live zebrafish embryos supports hindered diffusion model},
  volume={13},
  journal={Nature Communications},
  author={Kuhn, Timo and Landge, Amit N. and Mörsdorf, David and Coßmann, Jonas and Gerstenecker, Johanna and Capek, Daniel and Müller, Patrick and Gebhardt, J. Christof M.},
  note={Article Number: 6101}
}

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    <dc:creator>Landge, Amit N.</dc:creator>
    <dc:contributor>Müller, Patrick</dc:contributor>
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    <dcterms:abstract xml:lang="eng">The hindered diffusion model postulates that the movement of a signaling molecule through an embryo is affected by tissue geometry and binding-mediated hindrance, but these effects have not been directly demonstrated in vivo. Here, we visualize extracellular movement and binding of individual molecules of the activator-inhibitor signaling pair Nodal and Lefty in live developing zebrafish embryos using reflected light-sheet microscopy. We observe that diffusion coefficients of molecules are high in extracellular cavities, whereas mobility is reduced and bound fractions are high within cell-cell interfaces. Counterintuitively, molecules nevertheless accumulate in cavities, which we attribute to the geometry of the extracellular space by agent-based simulations. We further find that Nodal has a larger bound fraction than Lefty and shows a binding time of tens of seconds. Together, our measurements and simulations provide direct support for the hindered diffusion model and yield insights into the nanometer-to-micrometer-scale mechanisms that lead to macroscopic signal dispersal.</dcterms:abstract>
    <dc:contributor>Kuhn, Timo</dc:contributor>
    <dcterms:title>Single-molecule tracking of Nodal and Lefty in live zebrafish embryos supports hindered diffusion model</dcterms:title>
    <dc:contributor>Coßmann, Jonas</dc:contributor>
    <dc:creator>Kuhn, Timo</dc:creator>
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    <dc:contributor>Gebhardt, J. Christof M.</dc:contributor>
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    <dc:creator>Gerstenecker, Johanna</dc:creator>
    <dc:contributor>Gerstenecker, Johanna</dc:contributor>
    <dc:creator>Coßmann, Jonas</dc:creator>
    <dc:creator>Müller, Patrick</dc:creator>
    <dc:creator>Capek, Daniel</dc:creator>
    <dc:creator>Gebhardt, J. Christof M.</dc:creator>
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    <dc:language>eng</dc:language>
    <dc:rights>Attribution 4.0 International</dc:rights>
    <dc:contributor>Landge, Amit N.</dc:contributor>
    <dc:contributor>Mörsdorf, David</dc:contributor>
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