Identification of JAK/STAT pathway Regulators : Insights from RNAi screens

Müller, Patrick; Boutros, Michael; Zeidler, Martin P.

Identification of JAK/STAT pathway Regulators : Insights from RNAi screens

Dateien

Mueller_2-1nuo8gly0pjlt7.pdfGröße: 682.96 KBDownloads: 57

Datum

2008

Autor:innen

Müller, Patrick

Boutros, Michael

Zeidler, Martin P.

Open Access-Veröffentlichung

Open Access Hybrid

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Seminars in Cell & Developmental Biology. Elsevier. 2008, 19(4), pp. 360-369. ISSN 1084-9521. eISSN 1096-3634. Available under: doi: 10.1016/j.semcdb.2008.06.001

Zusammenfassung

While many core JAK/STAT pathway components have been discovered in Drosophila via classical genetic approaches, the identification of pathway regulators has been more challenging. Recently two cell-based RNAi screens for JAK/STAT pathway regulators have been undertaken using libraries of double-stranded RNAs targeting a large proportion of the predicted Drosophila transcriptome. While both screens identified multiple regulators, only relatively few loci are common to both data sets. Here we compare the two screens and discuss these differences. Although many factors are likely to be contributory, differences in the assay design are of key importance. Low levels of stimulation favouring the identification of negative pathway regulators and high levels of stimulation favouring the identification of positively acting factors. Ultimately, the results from both screens are likely to be largely complementary and have identified a range of novel candidate regulators of JAK/STAT pathway activity as a starting point for new research directions in the future.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

RNAi, Genome-wide screen, Cell culture, Luciferase reporter, Drosophila

Zitieren

ISO 690

MÜLLER, Patrick, Michael BOUTROS, Martin P. ZEIDLER, 2008. Identification of JAK/STAT pathway Regulators : Insights from RNAi screens. In: Seminars in Cell & Developmental Biology. Elsevier. 2008, 19(4), pp. 360-369. ISSN 1084-9521. eISSN 1096-3634. Available under: doi: 10.1016/j.semcdb.2008.06.001

BibTex

@article{Muller2008-08Ident-56530,
  year={2008},
  doi={10.1016/j.semcdb.2008.06.001},
  title={Identification of JAK/STAT pathway Regulators : Insights from RNAi screens},
  number={4},
  volume={19},
  issn={1084-9521},
  journal={Seminars in Cell & Developmental Biology},
  pages={360--369},
  author={Müller, Patrick and Boutros, Michael and Zeidler, Martin P.}
}

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