@article{Obayashi2017Molec-42856,
  year={2017},
  doi={10.1016/j.celrep.2017.02.052},
  title={Molecular Landscape of the Ribosome Pre-initiation Complex during mRNA Scanning : Structural Role for eIF3c and Its Control by eIF5},
  number={11},
  volume={18},
  journal={Cell reports},
  pages={2651--2663},
  author={Obayashi, Eiji and Luna, Rafael E and Nagata, Takashi and Martin-Marcos, Pilar and Hiraishi, Hiroyuki and Singh, Chingakham Ranjit and Erzberger, Jan Peter and Zhang, Fan and Arthanari, Haribabu and Morris, Jacob and Stengel, Florian}
}

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    <dc:creator>Zhang, Fan</dc:creator>
    <dc:contributor>Singh, Chingakham Ranjit</dc:contributor>
    <dc:contributor>Zhang, Fan</dc:contributor>
    <dc:creator>Obayashi, Eiji</dc:creator>
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    <dc:creator>Nagata, Takashi</dc:creator>
    <dc:contributor>Luna, Rafael E</dc:contributor>
    <dc:creator>Morris, Jacob</dc:creator>
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    <dc:contributor>Martin-Marcos, Pilar</dc:contributor>
    <dc:contributor>Arthanari, Haribabu</dc:contributor>
    <dc:creator>Stengel, Florian</dc:creator>
    <dc:creator>Luna, Rafael E</dc:creator>
    <dc:rights>Attribution 4.0 International</dc:rights>
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    <dcterms:title>Molecular Landscape of the Ribosome Pre-initiation Complex during mRNA Scanning : Structural Role for eIF3c and Its Control by eIF5</dcterms:title>
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    <dc:contributor>Stengel, Florian</dc:contributor>
    <dc:contributor>Obayashi, Eiji</dc:contributor>
    <dc:contributor>Hiraishi, Hiroyuki</dc:contributor>
    <dcterms:abstract xml:lang="eng">During eukaryotic translation initiation, eIF3 binds the solvent-accessible side of the 40S ribosome and recruits the gate-keeper protein eIF1 and eIF5 to the decoding center. This is largely mediated by the N-terminal domain (NTD) of eIF3c, which can be divided into three parts: 3c0, 3c1, and 3c2. The N-terminal part, 3c0, binds eIF5 strongly but only weakly to the ribosome-binding surface of eIF1, whereas 3c1 and 3c2 form a stoichiometric complex with eIF1. 3c1 contacts eIF1 through Arg-53 and Leu-96, while 3c2 faces 40S protein uS15/S13, to anchor eIF1 to the scanning pre-initiation complex (PIC). We propose that the 3c0:eIF1 interaction diminishes eIF1 binding to the 40S, whereas 3c0:eIF5 interaction stabilizes the scanning PIC by precluding this inhibitory interaction. Upon start codon recognition, interactions involving eIF5, and ultimately 3c0:eIF1 association, facilitate eIF1 release. Our results reveal intricate molecular interactions within the PIC, programmed for rapid scanning-arrest at the start codon.</dcterms:abstract>
    <dc:contributor>Morris, Jacob</dc:contributor>
    <dcterms:issued>2017</dcterms:issued>
    <dc:creator>Singh, Chingakham Ranjit</dc:creator>
    <dc:creator>Hiraishi, Hiroyuki</dc:creator>
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    <dc:creator>Arthanari, Haribabu</dc:creator>
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    <dc:creator>Martin-Marcos, Pilar</dc:creator>
    <dc:contributor>Nagata, Takashi</dc:contributor>
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    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-07-13T12:31:38Z</dc:date>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/42856"/>
    <dc:contributor>Erzberger, Jan Peter</dc:contributor>
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