Calcium-Binding Capacity of Centrin2 Is Required for Linear POC5 Assembly but Not for Nucleotide Excision Repair

Dantas, Tiago J.; Daly, Owen M.; Conroy, Pauline C.; Tomas, Martin; Wang, Yifan; Lalor, Pierce; Dockery, Peter; Ferrando-May, Elisa; Morrison, Ciaran G.

Calcium-Binding Capacity of Centrin2 Is Required for Linear POC5 Assembly but Not for Nucleotide Excision Repair

Dateien

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Datum

2013

Autor:innen

Dantas, Tiago J.

Daly, Owen M.

Conroy, Pauline C.

Tomas, Martin

Wang, Yifan

Lalor, Pierce

Dockery, Peter

Ferrando-May, Elisa

Morrison, Ciaran G.

Open Access-Veröffentlichung

Open Access Gold

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

PLoS ONE. 2013, 8(7), e68487. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0068487

Zusammenfassung

Centrosomes, the principal microtubule-organising centres in animal cells, contain centrins, small, conserved calcium-binding proteins unique to eukaryotes. Centrin2 binds to xeroderma pigmentosum group C protein (XPC), stabilising it, and its presence slightly increases nucleotide excision repair (NER) activity in vitro. In previous work, we deleted all three centrin isoforms present in chicken DT40 cells and observed delayed repair of UV-induced DNA lesions, but no centrosome abnormalities. Here, we explore how centrin2 controls NER. In the centrin null cells, we expressed centrin2 mutants that cannot bind calcium or that lack sites for phosphorylation by regulatory kinases. Expression of any of these mutants restored the UV sensitivity of centrin null cells to normal as effectively as expression of wild-type centrin. However, calcium-binding-deficient and T118A mutants showed greatly compromised localisation to centrosomes. XPC recruitment to laser-induced UV-like lesions was only slightly slower in centrin-deficient cells than in controls, and levels of XPC and its partner HRAD23B were unaffected by centrin deficiency. Interestingly, we found that overexpression of the centrin interactor POC5 leads to the assembly of linear, centrin-dependent structures that recruit other centrosomal proteins such as PCM-1 and NEDD1. Together, these observations suggest that assembly of centrins into complex structures requires calcium binding capacity, but that such assembly is not required for centrin activity in NER.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

DANTAS, Tiago J., Owen M. DALY, Pauline C. CONROY, Martin TOMAS, Yifan WANG, Pierce LALOR, Peter DOCKERY, Elisa FERRANDO-MAY, Ciaran G. MORRISON, 2013. Calcium-Binding Capacity of Centrin2 Is Required for Linear POC5 Assembly but Not for Nucleotide Excision Repair. In: PLoS ONE. 2013, 8(7), e68487. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0068487

BibTex

@article{Dantas2013-07-02Calci-42047,
  year={2013},
  doi={10.1371/journal.pone.0068487},
  title={Calcium-Binding Capacity of Centrin2 Is Required for Linear POC5 Assembly but Not for Nucleotide Excision Repair},
  number={7},
  volume={8},
  journal={PLoS ONE},
  author={Dantas, Tiago J. and Daly, Owen M. and Conroy, Pauline C. and Tomas, Martin and Wang, Yifan and Lalor, Pierce and Dockery, Peter and Ferrando-May, Elisa and Morrison, Ciaran G.},
  note={Article Number: e68487}
}

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