A mass spectrometry-based hybrid method for structural modeling of protein complexes

Politis, Argyris; Stengel, Florian; Hall, Zoe; Hernández, Helena; Leitner, Alexander; Walzthoeni, Thomas; Robinson, Carol V.; Aebersold, Ruedi

A mass spectrometry-based hybrid method for structural modeling of protein complexes

Dateien

Politis_2-sizb2igmjna15.pdfGröße: 352.25 KBDownloads: 250

Datum

2014

Autor:innen

Politis, Argyris

Stengel, Florian

Hall, Zoe

Hernández, Helena

Leitner, Alexander

Walzthoeni, Thomas

Robinson, Carol V.

Aebersold, Ruedi

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Nature Methods. 2014, 11(4), pp. 403-406. ISSN 1548-7091. eISSN 1548-7105. Available under: doi: 10.1038/nmeth.2841

Zusammenfassung

We describe a method that integrates data derived from different mass spectrometry (MS)-based techniques with a modeling strategy for structural characterization of protein assemblies. We encoded structural data derived from native MS, bottom-up proteomics, ion mobility-MS and chemical cross-linking MS into modeling restraints to compute the most likely structure of a protein assembly. We used the method to generate near-native models for three known structures and characterized an assembly intermediate of the proteasomal base.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

POLITIS, Argyris, Florian STENGEL, Zoe HALL, Helena HERNÁNDEZ, Alexander LEITNER, Thomas WALZTHOENI, Carol V. ROBINSON, Ruedi AEBERSOLD, 2014. A mass spectrometry-based hybrid method for structural modeling of protein complexes. In: Nature Methods. 2014, 11(4), pp. 403-406. ISSN 1548-7091. eISSN 1548-7105. Available under: doi: 10.1038/nmeth.2841

BibTex

@article{Politis2014spect-33030,
  year={2014},
  doi={10.1038/nmeth.2841},
  title={A mass spectrometry-based hybrid method for structural modeling of protein complexes},
  number={4},
  volume={11},
  issn={1548-7091},
  journal={Nature Methods},
  pages={403--406},
  author={Politis, Argyris and Stengel, Florian and Hall, Zoe and Hernández, Helena and Leitner, Alexander and Walzthoeni, Thomas and Robinson, Carol V. and Aebersold, Ruedi}
}

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