Conventional cell culture media do not adequately supply cells with antioxidants and thus facilitate peroxide-induced genotoxicity

Leist, Marcel; Raab, Barbara; Maurer, Stefanie; Rösick, Ullrich; Brigelius-Flohé, Regina

Conventional cell culture media do not adequately supply cells with antioxidants and thus facilitate peroxide-induced genotoxicity

Dateien

Leist_Conventional.pdfGröße: 1.47 MBDownloads: 1264

Datum

1996

Autor:innen

Leist, Marcel

Raab, Barbara

Maurer, Stefanie

Rösick, Ullrich

Brigelius-Flohé, Regina

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Free Radical Biology and Medicine. 1996, 21(3), pp. 297-306. ISSN 0891-5849. Available under: doi: 10.1016/0891-5849(96)00045-7

Zusammenfassung

Commercially available calf serum did not supply the cultured murine fibroblast cell line L929 with amounts of selenium and α-tocopherol sufficient to protect against peroxide damage. Supplementation of the culture medium with 30μM α-tocopherol or 50 nM sodium selenite led to a substantial increase of cellular α-tocopherol concentrations from 18 ± 3.0 to 3179 ±93.0 pmol/106 cells or cellular selenium concentrations from 0.17 ±0.02 to 1.75 ±0.16 ng/106 cells, respectively. L929 fibroblasts grown in selenite-containing medium also had markedly raised activities of both cytosolic glutathione peroxidase (from 11 ±0.9 to 67.2 ±4.2 mU/107 cells) and phospholipid hydroperoxide glutathione peroxidase (from 0.2 to 9.5 ±0.9 mU/107 cells). Supplementation with α-tocopherol inhibited single-strand breaks induced by low concentrations of H2O2 only, whereas an adequate selenium supply almost completely inhibited single-strand breaks induced by up to 30μM H2O2 and also significantly reduced H2O2induced cell death. An inadequate selenium supply and corresponding increase of GPx activity upon selenite supplementation was also observed with other cell lines, for instance, D10N, ECV-304, HepG2, and THP-1. Our data strengthen the relevance of standardized and adequate supplementation of tissue culture media with antioxidants to improve viability and genetic stability of cultured cells in general and in particular, if they are oxidatively challenged.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Cell culture, Selenium supply, Comet assay, DNA single strand breaks, Glutathione peroxidase, α-Tocopherol, Free radicals

Zitieren

ISO 690

LEIST, Marcel, Barbara RAAB, Stefanie MAURER, Ullrich RÖSICK, Regina BRIGELIUS-FLOHÉ, 1996. Conventional cell culture media do not adequately supply cells with antioxidants and thus facilitate peroxide-induced genotoxicity. In: Free Radical Biology and Medicine. 1996, 21(3), pp. 297-306. ISSN 0891-5849. Available under: doi: 10.1016/0891-5849(96)00045-7

BibTex

@article{Leist1996Conve-20165,
  year={1996},
  doi={10.1016/0891-5849(96)00045-7},
  title={Conventional cell culture media do not adequately supply cells with antioxidants and thus facilitate peroxide-induced genotoxicity},
  number={3},
  volume={21},
  issn={0891-5849},
  journal={Free Radical Biology and Medicine},
  pages={297--306},
  author={Leist, Marcel and Raab, Barbara and Maurer, Stefanie and Rösick, Ullrich and Brigelius-Flohé, Regina}
}

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