Interactions of bacteria with different mechanisms for chitin degradation result in the formation of a mixed-species biofilm

Jagmann, Nina; Styp von Rekowski, Katharina; Philipp, Bodo

Interactions of bacteria with different mechanisms for chitin degradation result in the formation of a mixed-species biofilm

Dateien

jagmann_214158.pdfGröße: 3.58 MBDownloads: 709

Datum

2012

Autor:innen

Jagmann, Nina

Styp von Rekowski, Katharina

Philipp, Bodo

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

FEMS Microbiology Letters. 2012, 326(1), pp. 69-75. ISSN 0378-1097. eISSN 1574-6968. Available under: doi: 10.1111/j.1574-6968.2011.02435.x

Zusammenfassung

In this study, interactions between bacteria possessing either released or cell-associated enzymes for polymer degradation were investigated. For this, a co-culture of Aeromonas hydrophila strain AH-1N as an enzyme-releasing bacterium and of Flavobacterium sp. strain 4D9 as a bacterium with cell-associated enzymes was set up with chitin embedded into agarose beads to account for natural conditions, under which polymers are usually embedded in organic aggregates. In single cultures, strain AH-1N grew with embedded chitin, while strain 4D9 did not. In co-cultures, strain 4D9 grew and outcompeted strain AH-1N in the biofilm fraction. Experiments with cell-free culture supernatants containing the chitinolytic enzymes of strain AH-1N revealed that growth of strain 4D9 in the co-culture was based on intercepting N-acetylglucosamine from chitin degradation. For this, strain 4D9 had to actively integrate into the biofilm of strain AH-1N. This study shows that bacteria using different chitin degradation mechanisms can coexist by formation of a mixed-species biofilm.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Aeromonas hydrophila, Flavobacterium, chitin, biofilm, interspecies interactions

Zitieren

ISO 690

JAGMANN, Nina, Katharina STYP VON REKOWSKI, Bodo PHILIPP, 2012. Interactions of bacteria with different mechanisms for chitin degradation result in the formation of a mixed-species biofilm. In: FEMS Microbiology Letters. 2012, 326(1), pp. 69-75. ISSN 0378-1097. eISSN 1574-6968. Available under: doi: 10.1111/j.1574-6968.2011.02435.x

BibTex

@article{Jagmann2012-01Inter-21415,
  year={2012},
  doi={10.1111/j.1574-6968.2011.02435.x},
  title={Interactions of bacteria with different mechanisms for chitin degradation result in the formation of a mixed-species biofilm},
  number={1},
  volume={326},
  issn={0378-1097},
  journal={FEMS Microbiology Letters},
  pages={69--75},
  author={Jagmann, Nina and Styp von Rekowski, Katharina and Philipp, Bodo}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/21415">
    <dc:creator>Philipp, Bodo</dc:creator>
    <dc:creator>Styp von Rekowski, Katharina</dc:creator>
    <dcterms:issued>2012-01</dcterms:issued>
    <dc:creator>Jagmann, Nina</dc:creator>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/21415/1/jagmann_214158.pdf"/>
    <dc:contributor>Styp von Rekowski, Katharina</dc:contributor>
    <dcterms:bibliographicCitation>FEMS Microbiology Letters ; 326 (2012), 1. - S. 69-75</dcterms:bibliographicCitation>
    <dc:contributor>Jagmann, Nina</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/21415/1/jagmann_214158.pdf"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-02-05T09:08:15Z</dc:date>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/21415"/>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Philipp, Bodo</dc:contributor>
    <dcterms:abstract xml:lang="eng">In this study, interactions between bacteria possessing either released or cell-associated enzymes for polymer degradation were investigated. For this, a co-culture of Aeromonas hydrophila strain AH-1N as an enzyme-releasing bacterium and of Flavobacterium sp. strain 4D9 as a bacterium with cell-associated enzymes was set up with chitin embedded into agarose beads to account for natural conditions, under which polymers are usually embedded in organic aggregates. In single cultures, strain AH-1N grew with embedded chitin, while strain 4D9 did not. In co-cultures, strain 4D9 grew and outcompeted strain AH-1N in the biofilm fraction. Experiments with cell-free culture supernatants containing the chitinolytic enzymes of strain AH-1N revealed that growth of strain 4D9 in the co-culture was based on intercepting N-acetylglucosamine from chitin degradation. For this, strain 4D9 had to actively integrate into the biofilm of strain AH-1N. This study shows that bacteria using different chitin degradation mechanisms can coexist by formation of a mixed-species biofilm.</dcterms:abstract>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-02-05T09:08:15Z</dcterms:available>
    <dcterms:title>Interactions of bacteria with different mechanisms for chitin degradation result in the formation of a mixed-species biofilm</dcterms:title>
    <dc:language>eng</dc:language>
  </rdf:Description>
</rdf:RDF>