Prostaglandin E(2) enhances T-cell proliferation by inducing the costimulatory molecules OX40L, CD70, and 4-1BBL on dendritic cells

Krause, Petra; Bruckner, Markus; Uermösi, Christina; Singer, Eva; Gröttrup, Marcus; Legler, Daniel F.

Prostaglandin E(2) enhances T-cell proliferation by inducing the costimulatory molecules OX40L, CD70, and 4-1BBL on dendritic cells

Dateien

Krause_opus-107550.pdfGröße: 497.04 KBDownloads: 552

Datum

2009

Autor:innen

Krause, Petra

Bruckner, Markus

Uermösi, Christina

Singer, Eva

Gröttrup, Marcus

Legler, Daniel F.

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie
Zukunftskolleg

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Blood. 2009, 113(11), pp. 2451-2460. ISSN 0006-4971. eISSN 1528-0020. Available under: doi: 10.1182/blood-2008-05-157123

Zusammenfassung

Dendritic cell (DC) based immunotherapy of malignant diseases relies on 2 critical parameters: antigen transport from the periphery to draining lymph nodes and efficient priming of primary and stimulation of secondary immune responses. Prostaglandin E2 (PGE2) signaling has been shown to be pivotal for DC migration toward lymph node derived chemokines in vitro and in vivo. Here, we demonstrate that PGE2 induced the expression of the costimulatory molecules OX40L, CD70, and 4-1BBL on human DCs. Short triggering by PGE2 early during DC maturation was sufficient to induce the costimulatory molecules. The expression of the costimulatory molecules was independent of the maturation stimulus but strictly dependent on PGE2 on both monocyte-derived (Mo) DCs and peripheral blood myeloid (PB) DCs. PGE2-matured MoDCs showed enhanced costimulatory capacities resulting in augmented antigen-specific CD4+ and CD8+ T-cell proliferation in primary and recall T-cell responses. Blocking OX40/OX40L signaling impaired the enhanced T-cell proliferation induced by PGE2-matured MoDCs. Moreover, MoDCs matured in the presence of PGE2 induced the expression of OX40, OX40L, and CD70 on T cells facilitating T-cell/T-cell interaction that warrant long-lasting costimulation. This newly identified parameter will help to further optimize DC-based immunotherapy.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

KRAUSE, Petra, Markus BRUCKNER, Christina UERMÖSI, Eva SINGER, Marcus GRÖTTRUP, Daniel F. LEGLER, 2009. Prostaglandin E(2) enhances T-cell proliferation by inducing the costimulatory molecules OX40L, CD70, and 4-1BBL on dendritic cells. In: Blood. 2009, 113(11), pp. 2451-2460. ISSN 0006-4971. eISSN 1528-0020. Available under: doi: 10.1182/blood-2008-05-157123

BibTex

@article{Krause2009Prost-1143,
  year={2009},
  doi={10.1182/blood-2008-05-157123},
  title={Prostaglandin E(2) enhances T-cell proliferation by inducing the costimulatory molecules OX40L, CD70, and 4-1BBL on dendritic cells},
  number={11},
  volume={113},
  issn={0006-4971},
  journal={Blood},
  pages={2451--2460},
  author={Krause, Petra and Bruckner, Markus and Uermösi, Christina and Singer, Eva and Gröttrup, Marcus and Legler, Daniel F.}
}

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