A complete citric acid cycle in assimilatory metabolism of Pelobacter acidigallici, a strictly anaerobic, fermenting bacterium

Brune, Andreas; Schink, Bernhard

A complete citric acid cycle in assimilatory metabolism of Pelobacter acidigallici, a strictly anaerobic, fermenting bacterium

Dateien

A_complete_citric_acid_cycle_in_assimilatory_1990.pdfGröße: 1.11 MBDownloads: 490

Datum

1990

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Archives of Microbiology. 1990, 154(4), pp. 394-399. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00276537

Zusammenfassung

Pelobacter acidigallici is a strictly anaerobic bacterium that ferments trihydroxybenzenes to 3 mol acetate/mol substrate. The key intermediate linking the catabolic sequences to the formation of cell matter is acetyl-CoA. Since P. acidigallici is independent of further external electron donors, it must oxidize part of the acetyl-CoA to provide reducing equivalents for anabolism. In this study we demonstrate the presence of all enzymes necessary to operate a modified citric acid cycle, with activities sufficient to support growth. Unusual enzymes in the cycle are 2-oxoglutarate synthase and succinyl- CoA:acetoacetate CoA transferase. Anaplerotic reactions are catalyzed by pyruvate synthase, PEP synthetase and PEP carboxylase. No CO dehydrogenase, hydrogenase, or formate dehydrogenase activity could be detected. The phylogenetic implications of these findings with respect to the relatedness of P. acidigallici to gramnegative, sulfur-reducing bacteria by 16S rRNA cataloguing are discussed.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Trihydroxybenzenes, Anabolism, Anaerobic citric acid cycle, Succinyl-CoA: acetoacetate CoA transferase, Phylogeny, Gram-negative bacteria

Zitieren

ISO 690

BRUNE, Andreas, Bernhard SCHINK, 1990. A complete citric acid cycle in assimilatory metabolism of Pelobacter acidigallici, a strictly anaerobic, fermenting bacterium. In: Archives of Microbiology. 1990, 154(4), pp. 394-399. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00276537

BibTex

@article{Brune1990compl-8625,
  year={1990},
  doi={10.1007/BF00276537},
  title={A complete citric acid cycle in assimilatory metabolism of Pelobacter acidigallici, a strictly anaerobic, fermenting bacterium},
  number={4},
  volume={154},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={394--399},
  author={Brune, Andreas and Schink, Bernhard}
}

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