Enzymes involved in anaerobic degradation of acetone by a denitrifying bacterium

Platen, Harald; Schink, Bernhard

Enzymes involved in anaerobic degradation of acetone by a denitrifying bacterium

Dateien

Enzymes_involved_in_anaerobic_1990.pdfGröße: 627.5 KBDownloads: 490

Datum

1990

Autor:innen

Platen, Harald

Schink, Bernhard

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Biodegradation. 1990, 1(4), pp. 243-251. ISSN 0923-9820. eISSN 1572-9729. Available under: doi: 10.1007/BF00119761

Zusammenfassung

The pathway of anaerobic acetone degradation by the denitrifying bacterial strain BunN was studied by enzyme measurements in extracts of anaerobic acetone-grown cells. An ADP- and MgC12-dependent decarboxylation of acetoacetate was detected which could not be found in cell-free extracts of acetate-grown cells. It is concluded that free acetoacetate is formed by ATP-dependent arboxylation of acetone. Acetoacetate was converted into its coenzyme A ester by succinyl-CoA: acetoacetate CoA transferase, and cleaved by a thiolase into acetyl-CoA. The acetyl residue was completely oxidized in the citric acid cycle. The ADP-dependent decarboxylation of acetoacetate was inhibited by EDTA, but not by avidin. High myokinase activities led to equilibrium amounts of ATP, ADP, and AMP in the reaction mixtures, and prevented determination of the decarboxylase reaction stoichiometry, therefore.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

carboxylation, decarboxylation, denitrification, metabolic pathway

Zitieren

ISO 690

PLATEN, Harald, Bernhard SCHINK, 1990. Enzymes involved in anaerobic degradation of acetone by a denitrifying bacterium. In: Biodegradation. 1990, 1(4), pp. 243-251. ISSN 0923-9820. eISSN 1572-9729. Available under: doi: 10.1007/BF00119761

BibTex

@article{Platen1990Enzym-6809,
  year={1990},
  doi={10.1007/BF00119761},
  title={Enzymes involved in anaerobic degradation of acetone by a denitrifying bacterium},
  number={4},
  volume={1},
  issn={0923-9820},
  journal={Biodegradation},
  pages={243--251},
  author={Platen, Harald and Schink, Bernhard}
}

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