Enzymes Involved in Anaerobic Polyethylene Glycol Degradation by Pelobacter venetianus and Bacteroides Strain PG1

Frings, Joachim; Schramm, Edgar; Schink, Bernhard

Enzymes Involved in Anaerobic Polyethylene Glycol Degradation by Pelobacter venetianus and Bacteroides Strain PG1

Dateien

Enzymes_Involved_in_Anaerobic_Polyethylene_Glycol_Degradation_1992.pdfGröße: 811.54 KBDownloads: 972

Datum

1992

Autor:innen

Frings, Joachim

Schramm, Edgar

Schink, Bernhard

URI (zitierfähiger Link)

http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-26888

Link zur Lizenz

Attribution-NonCommercial-NoDerivs 2.0 Generic

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Applied and Environmental Microbiology. 1992, 58(7), pp. 2164-2167

Zusammenfassung

In extracts of polyethylene glycol (PEG)-grown cells of the strictly anaerobically fermenting bacterium Pelobacter venetianus, two different enzyme activities were detected, a diol dehydratase and a PEG-degrading enzyme which was characterized as a PEG acetaldehyde lyase. Both enzymes were oxygen sensitive and depended on a reductant, such as titanium citrate or sulfhydryl compounds, for optimal activity. The diol dehydratase was inhibited by various corrinoids (adenosylcobalamin, cyanocobalamin, hydroxocobalamin, and methylcobalamin) by up to 37% at a concentration of 100 ,uM. Changes in ionic strength and the K+ ion concentration had only limited effects on this enzyme activity; glycerol inhibited the enzyme by 95%. The PEG-degrading enzyme activity was stimulated by the same corrinoids by up to 80%o, exhibited optimal activity in 0.75 M potassium phosphate buffer or in the presence of 4 M KCI, and was only slightly affected by glycerol. Both enzymes were located in the cytoplasmic space. Also, another PEG-degrading bacterium, Bacteroides strain PG1, contained a PEG acetaldehyde lyase activity analogous to the corresponding enzyme of P. venetianus but no diol dehydratase. Our results confirm that corrinoid-influenced PEG degradation analogous to a diol dehydratase reaction is a common strategy among several different strictly anaerobic PEG-degrading bacteria.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

FRINGS, Joachim, Edgar SCHRAMM, Bernhard SCHINK, 1992. Enzymes Involved in Anaerobic Polyethylene Glycol Degradation by Pelobacter venetianus and Bacteroides Strain PG1. In: Applied and Environmental Microbiology. 1992, 58(7), pp. 2164-2167

BibTex

@article{Frings1992Enzym-7185,
  year={1992},
  title={Enzymes Involved in Anaerobic Polyethylene Glycol Degradation by Pelobacter venetianus and Bacteroides Strain PG1},
  number={7},
  volume={58},
  journal={Applied and Environmental Microbiology},
  pages={2164--2167},
  author={Frings, Joachim and Schramm, Edgar and Schink, Bernhard}
}

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