Plasma Membrane H+-ATPase Activity in Spores,Germ Tubes, and Haustoria of the Rust Fungus Uromyces viciae-fabae

Struck, Christine; Hahn, Matthias; Mendgen, Kurt

Plasma Membrane H+-ATPase Activity in Spores,Germ Tubes, and Haustoria of the Rust Fungus Uromyces viciae-fabae

Dateien

159_struck96.pdfGröße: 666.44 KBDownloads: 418

Datum

1996

Autor:innen

Struck, Christine

Hahn, Matthias

Mendgen, Kurt

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Fungal Genetics and Biology. 1996, 20(1), pp. 30-35. ISSN 1087-1845. Available under: doi: 10.1006/fgbi.1996.0006

Zusammenfassung

Using plasma membrane-enriched vesicles, the properties of the H+-ATPase (EC 3.6.1.35) from the rust fungus Uromyces viciae-fabae were studied. The enzyme is strictly Mg2+-dependent and is inhibited by vanadate. The pH-optimum is at 6.7. By Western blot analysis using a monoclonal antibody against corn plasma membrane H+-ATPase a polypeptide of approximately 104 kDa could be detected. The vanadate-sensitive H+-ATPase activity of microsomal vesicles obtained from different stages of rust development was determined. Uredospores had only a very low enzyme activity (1.9 mmol Pi x mg-1 protein x h-1). In germ tubes the ATPase activity was about twofold higher (4.0 mmol Pi x mg-1 protein x h-1). An eightfold higher ATPase activity (16.1 mmol Pi x mg-1 protein x h-1) was found in microsomal vesicles from haustoria which had been isolated from rust-infected Vicia faba leaves. These results suggest, that the electrochemical gradient generated by the H+-ATPase of haustoria plays an important role for their function, possibly by promoting nutrient uptake from host cells.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

ATPase, biotrophic fungi, haustorium

Zitieren

ISO 690

STRUCK, Christine, Matthias HAHN, Kurt MENDGEN, 1996. Plasma Membrane H+-ATPase Activity in Spores,Germ Tubes, and Haustoria of the Rust Fungus Uromyces viciae-fabae. In: Fungal Genetics and Biology. 1996, 20(1), pp. 30-35. ISSN 1087-1845. Available under: doi: 10.1006/fgbi.1996.0006

BibTex

@article{Struck1996Plasm-6864,
  year={1996},
  doi={10.1006/fgbi.1996.0006},
  title={Plasma Membrane H+-ATPase Activity in Spores,Germ Tubes, and Haustoria of the Rust Fungus Uromyces viciae-fabae},
  number={1},
  volume={20},
  issn={1087-1845},
  journal={Fungal Genetics and Biology},
  pages={30--35},
  author={Struck, Christine and Hahn, Matthias and Mendgen, Kurt}
}

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