Low temperature or GroEL/ES overproduction permits growth of Escherichia coli cells lacking trigger factor and DnaK

Vorderwülbecke, Sonja; Kramer, Günter; Merz, Frieder; Kurz, Thorben A.; Rauch, Thomas; Zachmann-Brand, Beate; Bukau, Bernd; Deuerling, Elke

Low temperature or GroEL/ES overproduction permits growth of Escherichia coli cells lacking trigger factor and DnaK

Dateien

Low_temperature_or_GroEL.pdfGröße: 491.29 KBDownloads: 452

Datum

2004

Autor:innen

Vorderwülbecke, Sonja

Kramer, Günter

Merz, Frieder

Kurz, Thorben A.

Rauch, Thomas

Zachmann-Brand, Beate

Bukau, Bernd

Deuerling, Elke

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

FEBS Letters. 2004, 559(1-3), pp. 181-187. ISSN 0014-5793. Available under: doi: 10.1016/S0014-5793(04)00052-3

Zusammenfassung

Escherichia coli trigger factor (TF) and DnaK cooperate in the folding of newly synthesized proteins. The combined deletion of the TF-encoding tig gene and the dnaK gene causes protein aggregation and synthetic lethality at 30°C. Here we show that the synthetic lethality of ΔtigΔdnaK52 cells is abrogated either by growth below 30°C or by overproduction of GroEL/GroES. At 23°C ΔtigΔdnaK52 cells were viable and showed only minor protein aggregation. Overproduction of GroEL/GroES, but not of other chaperones, restored growth of ΔtigΔdnaK52 cells at 30°C and suppressed protein aggregation including proteins ≥60kDa, which normally require TF and DnaK for folding. GroEL/GroES thus influences the folding of proteins previously identified as DnaK/TF substrates.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Chaperone, De novo folding, 2D gel electrophoresis, Nascent polypeptide, Protein aggregation

Zitieren

ISO 690

VORDERWÜLBECKE, Sonja, Günter KRAMER, Frieder MERZ, Thorben A. KURZ, Thomas RAUCH, Beate ZACHMANN-BRAND, Bernd BUKAU, Elke DEUERLING, 2004. Low temperature or GroEL/ES overproduction permits growth of Escherichia coli cells lacking trigger factor and DnaK. In: FEBS Letters. 2004, 559(1-3), pp. 181-187. ISSN 0014-5793. Available under: doi: 10.1016/S0014-5793(04)00052-3

BibTex

@article{Vorderwulbecke2004tempe-7841,
  year={2004},
  doi={10.1016/S0014-5793(04)00052-3},
  title={Low temperature or GroEL/ES overproduction permits growth of Escherichia coli cells lacking trigger factor and DnaK},
  number={1-3},
  volume={559},
  issn={0014-5793},
  journal={FEBS Letters},
  pages={181--187},
  author={Vorderwülbecke, Sonja and Kramer, Günter and Merz, Frieder and Kurz, Thorben A. and Rauch, Thomas and Zachmann-Brand, Beate and Bukau, Bernd and Deuerling, Elke}
}

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