The Plasma Membrane H+-ATPase from the Biotrophic Rust Fungus Uromyces fabae : molecular Characterization of the Gene (PMA1) and Functional Expression of the Enzyme in Yeast

Struck, Christine; Siebels, Claudia; Rommel, Oliver; Wernitz, Marcus; Hahn, Matthias

The Plasma Membrane H+-ATPase from the Biotrophic Rust Fungus Uromyces fabae : molecular Characterization of the Gene (PMA1) and Functional Expression of the Enzyme in Yeast

Dateien

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Datum

1998

Autor:innen

Struck, Christine

Siebels, Claudia

Rommel, Oliver

Wernitz, Marcus

Hahn, Matthias

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Molecular Plant-Microbe Interaction. 1998, 11(6), pp. 458-465. ISSN 0894-0282. Available under: doi: 10.1094/MPMI.1998.11.6.458

Zusammenfassung

To study the molecular basis of biotrophic nutrient uptake by plant parasitic rust fungi, the gene (Uf-PMA1) encoding the plasma membrane H+-ATPase from Uromyces fabae was isolated. Uf-PMA1 exists probably as a single gene. However, two nearly identical sequences were identified; the similarity apparently is due to two Uf-PMA1 alleles in the dikaryotic hyphae. Multiple Uf-PMA1 transcripts were observed during early rust development, and reduced amounts of a single Uf-PMA1 mRNA were observed in haustoria and rust-infected leaves. This is in contrast to elevated enzyme activity in haustoria compared to germinated spores (C. Struck, M. Hahn, and K. Mendgen. Fungal Genet. Biol. 20:30-35, 1996). Unexpectedly, the PMA1-encoded rust protein is more similar to H+-ATPases from plants (55% identity) than from ascomycetous fungi (36% identity). When the rust PMA1 cDNA was expressed in Saccharomyces cerevisiae, both the wild-type enzyme and a mutant derivative (D76) deleted for the 76 C-terminal amino acids were able to support growth of a yeast strain lacking its own H+-ATPases. Compared to the wild-type, the D76 mutant enzyme displayed increased affinity to ATP, a higher vanadate sensitivity, and a more alkaline pH optimum. These results indicate that the C-terminal region of the rust enzyme exhibits autoregulatory properties.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Uromyces viciae-fabae

Zitieren

ISO 690

STRUCK, Christine, Claudia SIEBELS, Oliver ROMMEL, Marcus WERNITZ, Matthias HAHN, 1998. The Plasma Membrane H+-ATPase from the Biotrophic Rust Fungus Uromyces fabae : molecular Characterization of the Gene (PMA1) and Functional Expression of the Enzyme in Yeast. In: Molecular Plant-Microbe Interaction. 1998, 11(6), pp. 458-465. ISSN 0894-0282. Available under: doi: 10.1094/MPMI.1998.11.6.458

BibTex

@article{Struck1998Plasm-7722,
  year={1998},
  doi={10.1094/MPMI.1998.11.6.458},
  title={The Plasma Membrane H+-ATPase from the Biotrophic Rust Fungus Uromyces fabae : molecular Characterization of the Gene (PMA1) and Functional Expression of the Enzyme in Yeast},
  number={6},
  volume={11},
  issn={0894-0282},
  journal={Molecular Plant-Microbe Interaction},
  pages={458--465},
  author={Struck, Christine and Siebels, Claudia and Rommel, Oliver and Wernitz, Marcus and Hahn, Matthias}
}

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Universitätsbibliographie

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