Kinetic Investigations of the Mechanism of the Rate-Determing Step of the Na+, K+ -ATPase Pump Cycle

Clarke, Ronald J.; Humphrey, Paul; Lüpfert, Christian; Apell, Hans-Jürgen; Cornelius, Flemming

Kinetic Investigations of the Mechanism of the Rate-Determing Step of the Na+, K+ -ATPase Pump Cycle

Dateien

Kinetic_Investigations_of_the_Mechanism.pdfGröße: 193.26 KBDownloads: 325

Datum

2003

Autor:innen

Clarke, Ronald J.

Humphrey, Paul

Lüpfert, Christian

Apell, Hans-Jürgen

Cornelius, Flemming

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Annals of the New York Academy of Science. 2003, 986(1), pp. 159-162. ISSN 0077-8923. eISSN 1749-6632. Available under: doi: 10.1111/j.1749-6632.2003.tb07154.x

Zusammenfassung

The kinetics of the E2->E1 conformational change of unphosphorylated Na+ ,K+ -ATPase from rabbit kidney were investigated via the stopped-flow technique using the fluorescent label RH421 (pH 7.4, 24°C). The enzyme was preequilibrated in a solution containing 25 mM histidine and 0.1 mM EDTA to initially stabilize the E2 conformation. On mixing enzyme with NaCl alone, tris-ATP alone, or NaCl and tris-ATP simultaneously, a fluorescence decrease was observed.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

stopped-flow, fluorescence, voltage-sensitive dye, rate constant, regulation

Zitieren

ISO 690

CLARKE, Ronald J., Paul HUMPHREY, Christian LÜPFERT, Hans-Jürgen APELL, Flemming CORNELIUS, 2003. Kinetic Investigations of the Mechanism of the Rate-Determing Step of the Na+, K+ -ATPase Pump Cycle. In: Annals of the New York Academy of Science. 2003, 986(1), pp. 159-162. ISSN 0077-8923. eISSN 1749-6632. Available under: doi: 10.1111/j.1749-6632.2003.tb07154.x

BibTex

@article{Clarke2003Kinet-7310,
  year={2003},
  doi={10.1111/j.1749-6632.2003.tb07154.x},
  title={Kinetic Investigations of the Mechanism of the Rate-Determing Step of the Na+, K+ -ATPase Pump Cycle},
  number={1},
  volume={986},
  issn={0077-8923},
  journal={Annals of the New York Academy of Science},
  pages={159--162},
  author={Clarke, Ronald J. and Humphrey, Paul and Lüpfert, Christian and Apell, Hans-Jürgen and Cornelius, Flemming}
}

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