SP-A Binding Sites on Bovine Alveolar Macrophages

Plaga, Sandra; Plattner, Helmut; Schlepper-Schaefer, Jutta

SP-A Binding Sites on Bovine Alveolar Macrophages

Dateien

Plattner_SP_A_Binding_1998.pdfGröße: 289.79 KBDownloads: 680

Datum

1998

Autor:innen

Plaga, Sandra

Plattner, Helmut

Schlepper-Schaefer, Jutta

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Experimental Cell Research. 1998, 245(1), pp. 116-122. ISSN 0014-4827. Available under: doi: 10.1006/excr.1998.4226

Zusammenfassung

Surfactant protein A (SP-A) binding to bovine alveolar macrophages was examined in order to characterize SP-A binding proteins on the cell surface and to isolate putative receptors from these cells that could be obtained in large amounts. Human SP-A, unlabeled or labeled with gold particles, was bound to freshly isolated macrophages and analyzed with ELISA or the transmission electron microscope. Binding of SP-A was inhibited by Ca2+chelation, by an excess of unlabeled SP-A, or by the presence of 20 mg/ml mannan. We conclude that bovine alveolar macrophages expose binding sites for SP-A that are specific and that depend on Ca2+and on mannose residues. For isolation of SP-A receptors with homologous SP-A as ligand we isolated SP-A from bovine lung lavage. SDS PAGE analysis of the purified SP-A showed a protein of 32 36 kDa. Functional integrity of the protein was demonstrated. Bovine SP-A bound to Dynabeads was used to isolate SP-A binding proteins. From the fractionated and blotted proteins of the receptor preparation two proteins bound SP-A in a Ca2+-dependent manner, a 40-kDa protein showing mannose dependency and a 210-kDa protein, showing no mannose sensitivity.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

surfactant protein A, SP-A receptors, bovine alveolar macrophages, mannose-dependent binding, lung

Zitieren

ISO 690

PLAGA, Sandra, Helmut PLATTNER, Jutta SCHLEPPER-SCHAEFER, 1998. SP-A Binding Sites on Bovine Alveolar Macrophages. In: Experimental Cell Research. 1998, 245(1), pp. 116-122. ISSN 0014-4827. Available under: doi: 10.1006/excr.1998.4226

BibTex

@article{Plaga1998Bindi-7154,
  year={1998},
  doi={10.1006/excr.1998.4226},
  title={SP-A Binding Sites on Bovine Alveolar Macrophages},
  number={1},
  volume={245},
  issn={0014-4827},
  journal={Experimental Cell Research},
  pages={116--122},
  author={Plaga, Sandra and Plattner, Helmut and Schlepper-Schaefer, Jutta}
}

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