Electron microscopical identification of 3,3',5,5'-tetramethylbenzidine-reacted horseradish peroxidase after retrograde axoplasmic transport

Stürmer, Claudia; Bielenberg, Katrin; Spatz, Wolfbernhard B.

Electron microscopical identification of 3,3',5,5'-tetramethylbenzidine-reacted horseradish peroxidase after retrograde axoplasmic transport

Dateien

Electron_microscopical_identification_of.pdfGröße: 2.09 MBDownloads: 1000

Datum

1981

Autor:innen

Stürmer, Claudia

Bielenberg, Katrin

Spatz, Wolfbernhard B.

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Neuroscience Letters. 1981, 23(1), pp. 1-5. ISSN 0304-3940. Available under: doi: 10.1016/0304-3940(81)90181-6

Zusammenfassung

Tetramethylbenzidine (TMB) histochemistry for retrogradely transported horseradish peroxidase (HRP) has been successfully applied in electron microscopical preparations in the guinea pig visual cortex. In ultrathin sections, the TMB-HRP reaction product appears as an accumulation of typical electron-dense crystals. These crystals were identified in the cytoplasm of labeled cells in the proximal portions of the dendrites, and in myelinated axons. It is demonstrated that the TMB-HRP method is well suitable to analyze the ultrastructure of labeled cells and their synaptology.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

STÜRMER, Claudia, Katrin BIELENBERG, Wolfbernhard B. SPATZ, 1981. Electron microscopical identification of 3,3',5,5'-tetramethylbenzidine-reacted horseradish peroxidase after retrograde axoplasmic transport. In: Neuroscience Letters. 1981, 23(1), pp. 1-5. ISSN 0304-3940. Available under: doi: 10.1016/0304-3940(81)90181-6

BibTex

@article{Sturmer1981Elect-6808,
  year={1981},
  doi={10.1016/0304-3940(81)90181-6},
  title={Electron microscopical identification of 3,3',5,5'-tetramethylbenzidine-reacted horseradish peroxidase after retrograde axoplasmic transport},
  number={1},
  volume={23},
  issn={0304-3940},
  journal={Neuroscience Letters},
  pages={1--5},
  author={Stürmer, Claudia and Bielenberg, Katrin and Spatz, Wolfbernhard B.}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/6808">
    <dc:creator>Spatz, Wolfbernhard B.</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6808/1/Electron_microscopical_identification_of.pdf"/>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dcterms:title>Electron microscopical identification of 3,3',5,5'-tetramethylbenzidine-reacted horseradish peroxidase after retrograde axoplasmic transport</dcterms:title>
    <dcterms:abstract xml:lang="deu">Tetramethylbenzidine (TMB) histochemistry for retrogradely transported horseradish peroxidase (HRP) has been successfully applied in electron microscopical preparations in the guinea pig visual cortex. In ultrathin sections, the TMB-HRP reaction product appears as an accumulation of typical electron-dense crystals. These crystals were identified in the cytoplasm of labeled cells in the proximal portions of the dendrites, and in myelinated axons. It is demonstrated that the TMB-HRP method is well suitable to analyze the ultrastructure of labeled cells and their synaptology.</dcterms:abstract>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:29:20Z</dc:date>
    <dc:contributor>Bielenberg, Katrin</dc:contributor>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6808/1/Electron_microscopical_identification_of.pdf"/>
    <dc:creator>Bielenberg, Katrin</dc:creator>
    <dcterms:bibliographicCitation>First publ. in: Neuroscience Letters 23 (1981), pp. 1-5</dcterms:bibliographicCitation>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6808"/>
    <dc:creator>Stürmer, Claudia</dc:creator>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dc:contributor>Stürmer, Claudia</dc:contributor>
    <dc:format>application/pdf</dc:format>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:language>eng</dc:language>
    <dcterms:issued>1981</dcterms:issued>
    <dc:contributor>Spatz, Wolfbernhard B.</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:29:20Z</dcterms:available>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Nein