Characterization of microcystin production in an Antarctic cyanobacterial mat community
Dateien
Datum
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
Cyanobacteria are well known for their production of non-ribosomal cyclic peptide toxins, including microcystin, in temperate and tropical regions, however, the production of these compounds in extremely cold environments is still largely unexplored. Therefore, we investigated the production of protein phosphatase inhibiting microcystins by Antarctic cyanobacteria. We have identified microcystin-LR and for the first time [d-Asp3] microcystin-LR by mass spectrometric analysis in Antarctic cyanobacteria. The microcystins were extracted from a benthic microbial community that was sampled from a meltwater pond (Fresh Pond, McMurdo Ice Shelf, Antarctica). The extracted cyanobacterial cyclic peptides were equivalent to 11.4 ng MC-LR per mg dry weight by semi-quantitative analyses using HPLC-DAD and the protein phosphatase inhibition assay. Furthermore, we were able to identify the presence of cyanobacterial non-ribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) genes in total DNA extracts from the mat community.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
JUNGBLUT, Anne-Dorothee, Stefan J. HÖGER, Doug MOUNTFORT, Bettina C. HITZFELD, Daniel R. DIETRICH, Brett A. NEILAN, 2006. Characterization of microcystin production in an Antarctic cyanobacterial mat community. In: Toxicon. 2006, 47(3), pp. 271-278. ISSN 0041-0101. Available under: doi: 10.1016/j.toxicon.2005.11.002BibTex
@article{Jungblut2006Chara-7077, year={2006}, doi={10.1016/j.toxicon.2005.11.002}, title={Characterization of microcystin production in an Antarctic cyanobacterial mat community}, number={3}, volume={47}, issn={0041-0101}, journal={Toxicon}, pages={271--278}, author={Jungblut, Anne-Dorothee and Höger, Stefan J. and Mountfort, Doug and Hitzfeld, Bettina C. and Dietrich, Daniel R. and Neilan, Brett A.} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7077"> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7077"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:31:19Z</dc:date> <dc:creator>Höger, Stefan J.</dc:creator> <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights> <dc:creator>Mountfort, Doug</dc:creator> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7077/1/Characterization_of_microcystin_production_in_an_Antarctic_cyanobacterial_mat_community.pdf"/> <dc:creator>Dietrich, Daniel R.</dc:creator> <dc:language>eng</dc:language> <dc:creator>Jungblut, Anne-Dorothee</dc:creator> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7077/1/Characterization_of_microcystin_production_in_an_Antarctic_cyanobacterial_mat_community.pdf"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:contributor>Höger, Stefan J.</dc:contributor> <dc:contributor>Mountfort, Doug</dc:contributor> <dcterms:bibliographicCitation>First publ. in: Toxicon 47 (2006), 3, pp. 271-278</dcterms:bibliographicCitation> <dc:creator>Hitzfeld, Bettina C.</dc:creator> <dcterms:issued>2006</dcterms:issued> <dc:creator>Neilan, Brett A.</dc:creator> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/> <foaf:homepage rdf:resource="http://localhost:8080/"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:31:19Z</dcterms:available> <dc:contributor>Dietrich, Daniel R.</dc:contributor> <dc:contributor>Hitzfeld, Bettina C.</dc:contributor> <dc:contributor>Jungblut, Anne-Dorothee</dc:contributor> <dc:contributor>Neilan, Brett A.</dc:contributor> <dcterms:abstract xml:lang="eng">Cyanobacteria are well known for their production of non-ribosomal cyclic peptide toxins, including microcystin, in temperate and tropical regions, however, the production of these compounds in extremely cold environments is still largely unexplored. Therefore, we investigated the production of protein phosphatase inhibiting microcystins by Antarctic cyanobacteria. We have identified microcystin-LR and for the first time [d-Asp3] microcystin-LR by mass spectrometric analysis in Antarctic cyanobacteria. The microcystins were extracted from a benthic microbial community that was sampled from a meltwater pond (Fresh Pond, McMurdo Ice Shelf, Antarctica). The extracted cyanobacterial cyclic peptides were equivalent to 11.4 ng MC-LR per mg dry weight by semi-quantitative analyses using HPLC-DAD and the protein phosphatase inhibition assay. Furthermore, we were able to identify the presence of cyanobacterial non-ribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) genes in total DNA extracts from the mat community.</dcterms:abstract> <dcterms:title>Characterization of microcystin production in an Antarctic cyanobacterial mat community</dcterms:title> <dc:format>application/pdf</dc:format> </rdf:Description> </rdf:RDF>