Isolation of a hydrogenase-cytochrome b complex from cytoplasmic membranes of Xanthobacter autotrophicus GZ 29

Schink, Bernhard

Isolation of a hydrogenase-cytochrome b complex from cytoplasmic membranes of Xanthobacter autotrophicus GZ 29

Dateien

1982_Schink_289_293.pdfGröße: 424.27 KBDownloads: 282

Datum

1982

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

FEMS Microbiology Letters. 1982, 13(3), pp. 289-293. ISSN 0378-1097. eISSN 1574-6968. Available under: doi: 10.1111/j.1574-6968.1982.tb08274.x

Zusammenfassung

The aerobic nitrogen-fixing hydrogen bacterium Xanthobacter autotrophicus strain GZ29 [1] can grow autotrophically with hydrogen, oxygen, carbon dioxide, and molecular nitrogen as sole sources of electrons, energy, carbon, and nitrogen. Carbon dioxide is fixed via the Calvin cycle, and for activation of hydrogen only a membrane-bound hydrogenase activity was detected which does not reduce NAD [2]. This is true also for many other aerobic hydrogen bacteria [2,3], and it is assumed that in these organisms the membrane-bound hydrogenase has to provide electrons for NAD reduction in a reversed electron transport as well as for energy conservation. In X. autotrophicus the hydrogenase beyond it has to supply electrons for reduction of nitrogen by nitrogenase at the redox potential of ferredoxin (around - 4 2 0 mV). For this reason the hydrogenase of X. autotrophicus should differ from the type of membrane-bound hydrogenase found in Alcaligenes eutrophus which reduces electron acceptors only at the redox potential of quinones [4]. It was also considered that even two different hydrogenases might be present, one reducing ferredoxin and one providing electrons for the respiratory chain. The involvement of ferredoxin in the nitrogen fixation process of X. autotrophicus GZ29 has recently been shown by electron transfer and regulation studies [5]. In the present study the membrane-bound hydrogenase system of S. autotrophicus is shown to be associated with a cytochrome b complex, and catalytic as well as molecular properties are described.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

SCHINK, Bernhard, 1982. Isolation of a hydrogenase-cytochrome b complex from cytoplasmic membranes of Xanthobacter autotrophicus GZ 29. In: FEMS Microbiology Letters. 1982, 13(3), pp. 289-293. ISSN 0378-1097. eISSN 1574-6968. Available under: doi: 10.1111/j.1574-6968.1982.tb08274.x

BibTex

@article{Schink1982Isola-7045,
  year={1982},
  doi={10.1111/j.1574-6968.1982.tb08274.x},
  title={Isolation of a hydrogenase-cytochrome b complex from cytoplasmic membranes of Xanthobacter autotrophicus GZ 29},
  number={3},
  volume={13},
  issn={0378-1097},
  journal={FEMS Microbiology Letters},
  pages={289--293},
  author={Schink, Bernhard}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7045">
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:language>eng</dc:language>
    <dc:creator>Schink, Bernhard</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:issued>1982</dcterms:issued>
    <dc:contributor>Schink, Bernhard</dc:contributor>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7045"/>
    <dc:format>application/pdf</dc:format>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:31:05Z</dcterms:available>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7045/1/1982_Schink_289_293.pdf"/>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:abstract xml:lang="eng">The aerobic nitrogen-fixing hydrogen bacterium Xanthobacter autotrophicus strain GZ29 [1] can grow autotrophically with hydrogen, oxygen, carbon dioxide, and molecular nitrogen as sole sources of electrons, energy, carbon, and nitrogen. Carbon dioxide is fixed via the Calvin cycle, and for activation of hydrogen only a membrane-bound hydrogenase activity was detected which does not reduce NAD [2]. This is true also for many other aerobic hydrogen bacteria [2,3], and it is assumed that in these organisms the membrane-bound hydrogenase has to provide electrons for NAD reduction in a reversed electron transport as well as for energy conservation. In X. autotrophicus the hydrogenase beyond it has to supply electrons for reduction of nitrogen by nitrogenase at the redox potential of ferredoxin (around - 4 2 0 mV). For this reason the hydrogenase of X. autotrophicus should differ from the type of membrane-bound hydrogenase found in Alcaligenes eutrophus which reduces electron acceptors only at the redox potential of quinones [4]. It was also considered that even two different hydrogenases might be present, one reducing ferredoxin and one providing electrons for the respiratory chain. The involvement of ferredoxin in the nitrogen fixation process of X. autotrophicus GZ29 has recently been shown by electron transfer and regulation studies [5]. In the present study the membrane-bound hydrogenase system of S. autotrophicus is shown to be associated with a cytochrome b complex, and catalytic as well as molecular properties are described.</dcterms:abstract>
    <dcterms:bibliographicCitation>First publ. in: FEMS Microbiology Letters 13 (1982), 3, pp. 289-293</dcterms:bibliographicCitation>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7045/1/1982_Schink_289_293.pdf"/>
    <dc:rights>terms-of-use</dc:rights>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:31:05Z</dc:date>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:title>Isolation of a hydrogenase-cytochrome b complex from cytoplasmic membranes of Xanthobacter autotrophicus GZ 29</dcterms:title>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Nein