@article{Providenti2001Comam-6764,
  year={2001},
  doi={10.1099/00221287-147-8-2157},
  title={Comamonas testosteroni BR6020 possesses a single genetic locus for extradiol cleavage of protocatechuate},
  number={8},
  volume={147},
  issn={1350-0872},
  journal={Microbiology},
  pages={2157--2167},
  author={Providenti, Miguel A. and Mampel, Jörg and McSween, Scott and Cook, Alasdair M. and Wyndham, R. Campbell}
}

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    <dc:contributor>Cook, Alasdair M.</dc:contributor>
    <dc:contributor>Providenti, Miguel A.</dc:contributor>
    <dcterms:title>Comamonas testosteroni BR6020 possesses a single genetic locus for extradiol cleavage of protocatechuate</dcterms:title>
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    <dc:contributor>Mampel, Jörg</dc:contributor>
    <dcterms:issued>2001</dcterms:issued>
    <dc:creator>McSween, Scott</dc:creator>
    <dcterms:bibliographicCitation>First publ. in: Microbiology 147 (2001), 8, pp. 2157-2167</dcterms:bibliographicCitation>
    <dc:creator>Wyndham, R. Campbell</dc:creator>
    <dc:creator>Cook, Alasdair M.</dc:creator>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dc:creator>Mampel, Jörg</dc:creator>
    <dc:contributor>McSween, Scott</dc:contributor>
    <dc:creator>Providenti, Miguel A.</dc:creator>
    <dc:contributor>Wyndham, R. Campbell</dc:contributor>
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    <dcterms:abstract xml:lang="eng">A key intermediate for biodegradation of various distinct aromatic growth substrates in Comamonas testosteroni is protocatechuate (Pca), which is metabolized by the 4,5-extradiol (meta) ring fission pathway. A locus harbouring genes from C. testosteroni BR6020 was cloned, dubbed pmd, which encodes the enzymes that degrade Pca. The identity of pmdAB, encoding respectively the alpha- and beta-subunit of the Pca ring-cleavage enzyme, was confirmed by N-terminal sequencing and molecular mass determination of both subunits from the separated enzyme. Disruption of pmdA resulted in a strain unable to grow on Pca and a variety of aromatic substrates funnelled through this compound (m- and p-hydroxybenzoate, p-sulfobenzoate, phthalate, isophthalate, terephthalate, vanillate, isovanillate and veratrate). Growth on benzoate and o-aminobenzoate (anthranilate) was not affected in this strain, indicating that these substrates are metabolized via a different lower pathway. Tentative functions for the products of other pmd genes were assigned based on sequence identity and/or similarity to proteins from other proteobacteria involved in uptake or metabolism of aromatic compounds. This study provides evidence for a single lower pathway in C. testosteroni for metabolism of Pca, which is generated by different upper pathways acting on a variety of aromatic substrates.</dcterms:abstract>
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