Presentation of lipoteichoic acid potentiates its inflammatory activity

Deininger, Susanne; Traub, Stephanie; Aichele, Diana; Rupp, Tamara; Baris, Teodora; Möller, Heiko M.; Hartung, Thomas; Aulock, Sonja von

Presentation of lipoteichoic acid potentiates its inflammatory activity

Dateien

Deininger_Immunobiol_2008.pdfGröße: 6.95 MBDownloads: 674

Datum

2008

Autor:innen

Deininger, Susanne

Traub, Stephanie

Aichele, Diana

Rupp, Tamara

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Chemie
Biologie
Zukunftskolleg

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Immunobiology. 2008, 213(6), pp. 519-529. ISSN 0171-2985. Available under: doi: 10.1016/j.imbio.2008.01.001

Zusammenfassung

Lipoteichoic acid (LTA) is a major immunostimulatory molecule in the cell wall of Gram-positive bacteria. Adhesion of LTA to a polystyrene surface drastically increased its immunostimulatory potency in human whole blood in comparison to soluble LTA, although only 1% of the LTA had bound, as determined using rhodamine-labelled LTA. The release of the proinflammatory cytokines IL-1beta, TNF and IL-6 and the chemokines IL-8 and G-CSF was increased 2- to 10-fold, but IL-10 release was unaltered. This presentation effect was not shared by lipopolysaccharide (LPS) or other toll-like receptor 2 agonists and was less pronounced in polypropylene vessels. LTA did not induce cytokine release in silicone-coated borosilicate vessels, but covalent coupling of LTA to polystyrene beads restored cytokine induction in these vessels, indicating that presentation of LTA on a surface is in fact essential for its immunostimulatory potency.
This novel aspect of presentation as a factor in the recognition of LTA may reflect the physiological situation in the bacterial cell wall, where LTA is anchored in the bacterial membrane and projects through the peptidoglycan. In practical terms, contamination of medical devices with components of Gram-positive bacteria may pose an underestimated inflammatory risk.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Bacteria, Cytokines, Inflammation, Lipoteichoic acid, Potency

Zitieren

ISO 690

DEININGER, Susanne, Stephanie TRAUB, Diana AICHELE, Tamara RUPP, Teodora BARIS, Heiko M. MÖLLER, Thomas HARTUNG, Sonja von AULOCK, 2008. Presentation of lipoteichoic acid potentiates its inflammatory activity. In: Immunobiology. 2008, 213(6), pp. 519-529. ISSN 0171-2985. Available under: doi: 10.1016/j.imbio.2008.01.001

BibTex

@article{Deininger2008Prese-9714,
  year={2008},
  doi={10.1016/j.imbio.2008.01.001},
  title={Presentation of lipoteichoic acid potentiates its inflammatory activity},
  number={6},
  volume={213},
  issn={0171-2985},
  journal={Immunobiology},
  pages={519--529},
  author={Deininger, Susanne and Traub, Stephanie and Aichele, Diana and Rupp, Tamara and Baris, Teodora and Möller, Heiko M. and Hartung, Thomas and Aulock, Sonja von}
}

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    <dcterms:abstract xml:lang="eng">Lipoteichoic acid (LTA) is a major immunostimulatory molecule in the cell wall of Gram-positive bacteria. Adhesion of LTA to a polystyrene surface drastically increased its immunostimulatory potency in human whole blood in comparison to soluble LTA, although only 1% of the LTA had bound, as determined using rhodamine-labelled LTA. The release of the proinflammatory cytokines IL-1beta, TNF and IL-6 and the chemokines IL-8 and G-CSF was increased 2- to 10-fold, but IL-10 release was unaltered. This presentation effect was not shared by lipopolysaccharide (LPS) or other toll-like receptor 2 agonists and was less pronounced in polypropylene vessels. LTA did not induce cytokine release in silicone-coated borosilicate vessels, but covalent coupling of LTA to polystyrene beads restored cytokine induction in these vessels, indicating that presentation of LTA on a surface is in fact essential for its immunostimulatory potency.&lt;br /&gt;This novel aspect of presentation as a factor in the recognition of LTA may reflect the physiological situation in the bacterial cell wall, where LTA is anchored in the bacterial membrane and projects through the peptidoglycan. In practical terms, contamination of medical devices with components of Gram-positive bacteria may pose an underestimated inflammatory risk.</dcterms:abstract>
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