@article{Dymalla2009novel-1248,
  year={2009},
  doi={10.1007/s00109-008-0432-1},
  title={A novel peptide motif binding to and blocking the intracellular activity of the human papillomavirus E6 oncoprotein},
  number={3},
  volume={87},
  issn={0946-2716},
  journal={Journal of Molecular Medicine},
  pages={321--331},
  author={Dymalla, Susanne and Scheffner, Martin and Weber, Elvira and Sehr, Peter and Lohrey, Claudia and Hoppe-Seyler, Felix and Hoppe-Seyler, Karin}
}

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    <dc:creator>Hoppe-Seyler, Karin</dc:creator>
    <dc:contributor>Dymalla, Susanne</dc:contributor>
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    <dc:contributor>Weber, Elvira</dc:contributor>
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    <dc:contributor>Hoppe-Seyler, Karin</dc:contributor>
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    <dc:creator>Sehr, Peter</dc:creator>
    <dc:contributor>Sehr, Peter</dc:contributor>
    <dc:creator>Scheffner, Martin</dc:creator>
    <dc:contributor>Hoppe-Seyler, Felix</dc:contributor>
    <dc:contributor>Lohrey, Claudia</dc:contributor>
    <dc:contributor>Scheffner, Martin</dc:contributor>
    <dc:language>eng</dc:language>
    <dcterms:title>A novel peptide motif binding to and blocking the intracellular activity of the human papillomavirus E6 oncoprotein</dcterms:title>
    <dc:creator>Hoppe-Seyler, Felix</dc:creator>
    <dc:rights>terms-of-use</dc:rights>
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    <dc:creator>Dymalla, Susanne</dc:creator>
    <dcterms:abstract>Specific types of human papillomaviruses (HPVs) cause cervical cancer. The viral E6 oncogene is a critical factor for maintaining the malignant phenotype of HPV-positive tumour cells. By yeast two-hybrid screening of a randomised peptide expression library, we isolated linear short peptides, which specifically bind to the HPV16 E6 oncoprotein. Sequence alignments and mutational analyses of the peptides identified a hitherto undiscovered E6-binding motif. Intracellular expression of a peptide containing the novel E6-binding motif resulted in inhibition of colony formation capacity, specifically of HPV16-positive cancer cells. A solubility-optimised variant of the peptide was created, which binds to HPV16 E6 with high affinity. Its intracellular expression efficiently induced apoptosis in HPV16-positive cancer cells. This was linked to restoration of intracellular p53 activities. Thus, this newly identified E6-binding motif could form a novel basis for the development of rational strategies for the treatment of HPV16-positive preneoplastic and neoplastic lesions.</dcterms:abstract>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:07:24Z</dc:date>
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    <dc:creator>Lohrey, Claudia</dc:creator>
    <dc:creator>Weber, Elvira</dc:creator>
    <dcterms:bibliographicCitation>First publ. in: Journal of Molecular Medicine 87 (2009), 3, pp. 321-331</dcterms:bibliographicCitation>
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