Synthesis of DNA Strands Site-Specifically Damaged by C8-Arylamine Purine Adducts and Effects on Various DNA Polymerases

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Boege_opus-86508.pdf
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2008
Autor:innen
Böge, Nicolas
Jacobsen, Maike I.
Szombati, Zita
Baerns, Sabrina
Di Pasquale, Francesca
Meier, Chris
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Chemistry : a European Journal. 2008, 14(35), pp. 11194-11208. ISSN 0947-6539. eISSN 1521-3765. Available under: doi: 10.1002/chem.200800979
Zusammenfassung

C8-Arylamine-dG and C8-arylamine-dA adducts have been prepared using palladium cross-coupling chemistry. These adducts were subsequently converted into the corresponding 5'-O-DMTr-C8-arylamine-3'-O-phosphoramidites and then used for the automated synthesis of different site-specifically modified oligonucleotides. These "damaged" oligonucleotides have been characterized by ESI-MS, UV thermal stability assays, and circular dichroism, and they have been used in EcoRI assays as well as in primer extension studies using various DNA polymerases.

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Fachgebiet (DDC)
540 Chemie
Schlagwörter
aromatic amines, cross-coupling, DNA adducts, DNA damage, palladium
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ISO 690BÖGE, Nicolas, Maike I. JACOBSEN, Zita SZOMBATI, Sabrina BAERNS, Francesca DI PASQUALE, Andreas MARX, Chris MEIER, 2008. Synthesis of DNA Strands Site-Specifically Damaged by C8-Arylamine Purine Adducts and Effects on Various DNA Polymerases. In: Chemistry : a European Journal. 2008, 14(35), pp. 11194-11208. ISSN 0947-6539. eISSN 1521-3765. Available under: doi: 10.1002/chem.200800979
BibTex
@article{Boge2008Synth-1019,
  year={2008},
  doi={10.1002/chem.200800979},
  title={Synthesis of DNA Strands Site-Specifically Damaged by C8-Arylamine Purine Adducts and Effects on Various DNA Polymerases},
  number={35},
  volume={14},
  issn={0947-6539},
  journal={Chemistry : a European Journal},
  pages={11194--11208},
  author={Böge, Nicolas and Jacobsen, Maike I. and Szombati, Zita and Baerns, Sabrina and Di Pasquale, Francesca and Marx, Andreas and Meier, Chris}
}
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