Immunological Investigation of Human Complement Receptor Type II (CR2/CD21) : Serum Soluble CD21 in Health and Disease

Masilamani, Madhan

Immunological Investigation of Human Complement Receptor Type II (CR2/CD21) : Serum Soluble CD21 in Health and Disease

Dateien

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Datum

2002

Autor:innen

Masilamani, Madhan

URI (zitierfähiger Link)

http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-9734

Link zur Lizenz

Urheberrechtlich geschützt

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Titel in einer weiteren Sprache

Immunologische Untersuchung des humanen Komplementrezeptors Typ II (CR2/CD21). Lösliches CD21 in Gesundheit und Krankheit

Publikationstyp

Dissertation

Publikationsstatus

Published

Zusammenfassung

In this study, the expression and regulation of membrane associated human complement receptor type-II (CR2/CD21) and its soluble form were investigated.
The expression of CD21 was analysed in normal and activated T cells. PCR analyses and immunofluorescence/confocal microscopy of peripheral blood T cells and of activated T cells showed reduction in CD21 mRNA and protein expression upon activation.
CD21 is released as a soluble form from the cell surface by proteolytic activity known as Shedding. Soluble CD21 was isolated and purified from human plasma to homogeneity applying affinity chromatography and density gradient centrifugation. Soluble CD21 was found to be a single 126 kDa molecular species. By determining the sedimentation coefficient of sCD21, the hydrodynamic properties such as diffusion coefficient and the frictional coefficient were calculated. These values show that the sCD21 isolated from human plasma is an elongated rod shaped molecule. A specific ELISA was developed using purified sCD21 as a standard for the determination of sCD21 concentration in human sera. Soluble CD21 levels were estimated in serum samples from 235 healthy donors, 209 RA patients, 17 patients with arthritis related disorders, 41 CVID patients, 10 healthy pregnant women and 10 neonates. The normal values of plasma sCD21 in healthy individuals of age between 20-40 years range from 100-500 ng/ml (median 292 ng/ml) with a tendency to decrease with age but did not differ with gender. sCD21 levels were significantly reduced in rheumatoid arthritis (P

Zusammenfassung in einer weiteren Sprache

Es wurde die Expression u. Regulation d, humanen membraneständigen Komplementrezeptors Type-II (CR2/CD21) u. seiner lösl. CD21-Form untersucht. Die Expression von CD21 wurde in normalen u. aktivierten T-Zellen analysiert. Mit Hilfe von PCR u. Immunofluoreszenz/konfokaler Mikroskopie von peripheren Blut-T-Zellen u. aktivierten T-Zellen wurde nach Aktivierung e. Reduktion in der CD21 mRNA u. in der Proteinexpression gezeigt. Es handelt sich dabei um ein einzelnes 126 kDa großes Molekül. Mit d. Bestimmung d. Sedimentationskoeffizienten von sCD21 wurden d. hydrodynam. Eigenschaften wie d. Diffusionskoeffizient u. der Reibungskoeffizient berechnet. Diese Werte zeigen, daß d. aus menschl. Serum isolierte sCD21 e. langgestrecktes routenförmiges Molekül ist. Der Gehalt an lösl. CD21 wurde in Seren von 235 gesunden Spendern, 209 RA-Patienten, 17 Patienten mit arthritisverwandten Krankheiten, 41 CVID Patienten, 10 gesunden schwangeren Frauen u. 10 Neugeborenen ermittelt. Der sCD21-Gehalt bei Schwangeren war signifikant reduziert u. erlangte auch kurz nach der Geburt nicht wieder seinen Normalwert. Der sCD21-Gehalt unterschied sich nicht signifikant zw. Mutter und Neugeborenem. Quelle u. Mechanismus d. CD21-Shedding wurden in primären Lymphozyten u. lymphoiden Zelllinien unters. In B-Zellen existiert ein höheres CD21-Shedding als in T-Zellen, daher tragen diese zum Plasma sCD21-Pool bei. Mitogen (PMA) Aktivierung von Lymphozyten induziert Shedding von CD21 u. korreliert mit Abnahme des CD21-Gehalts auf d. Zelloberfläche in Raji B-Zellen. Das Shedding wurde durch Alpha1-Antitrypsin, ein Serinproteasehemmer u. EDTA, ein Metalloproteasehemmer, inhibiert. Alpha1-Antitrypsin inhibierte auch das PMA-induzierte CD21-Shedding. Eine weitere Untergliederung d. Signaltransduktionsweges führt möglicherweise zur Identifizierung von neuen Mechanismen, die zum regulierten Shedding von CD21 führen u. somit zur Modulierung der Immunantwort.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

CR2/CD21, Isolierung, Rheumatoide Arthritis, CVID, Schwangerschaft, CR2/CD21, Rheumatoid Arthritis, CVID, Pregnancy

Zitieren

ISO 690

MASILAMANI, Madhan, 2002. Immunological Investigation of Human Complement Receptor Type II (CR2/CD21) : Serum Soluble CD21 in Health and Disease [Dissertation]. Konstanz: University of Konstanz

BibTex

@phdthesis{Masilamani2002Immun-8179,
  year={2002},
  title={Immunological Investigation of Human Complement Receptor Type II (CR2/CD21) : Serum Soluble CD21 in Health and Disease},
  author={Masilamani, Madhan},
  address={Konstanz},
  school={Universität Konstanz}
}

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Prüfungsdatum der Dissertation

January 27, 2003