Universität zu Köln

Perrier, Dayinta L., Vahid, Afshin, Kathavi, Vaishnavi, Stam, Lotte, Rems, Lea ORCID: 0000-0001-7470-4367, Mulla, Yuval, Muralidharan, Aswin ORCID: 0000-0003-2240-4260, Koenderink, Gijsje H., Kreutzer, Michiel T. and Boukany, Pouyan E. (2019). Response of an actin network in vesicles under electric pulses. Sci Rep, 9. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

We study the role of a biomimetic actin network during the application of electric pulses that induce electroporation or electropermeabilization, using giant unilamellar vesicles (GUVs) as a model system. The actin cortex, a subjacently attached interconnected network of actin filaments, regulates the shape and mechanical properties of the plasma membrane of mammalian cells, and is a major factor influencing the mechanical response of the cell to external physical cues. We demonstrate that the presence of an actin shell inhibits the formation of macropores in the electroporated GUVs. Additionally, experiments on the uptake of dye molecules after electroporation show that the actin network slows down the resealing process of the permeabilized membrane. We further analyze the stability of the actin network inside the GUVs exposed to high electric pulses. We find disruption of the actin layer that is likely due to the electrophoretic forces acting on the actin filaments during the permeabilization of the GUVs. Our findings on the GUVs containing a biomimetic network provide a step towards understanding the discrepancies between the electroporation mechanism of a living cell and its simplified model of the empty GUV.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Perrier, Dayinta L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Vahid, Afshin UNSPECIFIED UNSPECIFIED UNSPECIFIED Kathavi, Vaishnavi UNSPECIFIED UNSPECIFIED UNSPECIFIED Stam, Lotte UNSPECIFIED UNSPECIFIED UNSPECIFIED Rems, Lea UNSPECIFIED orcid.org/0000-0001-7470-4367 UNSPECIFIED Mulla, Yuval UNSPECIFIED UNSPECIFIED UNSPECIFIED Muralidharan, Aswin UNSPECIFIED orcid.org/0000-0003-2240-4260 UNSPECIFIED Koenderink, Gijsje H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kreutzer, Michiel T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Boukany, Pouyan E. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-147718
DOI:	10.1038/s41598-019-44613-5
Journal or Publication Title:	Sci Rep
Volume:	9
Date:	2019
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GIANT VESICLES; CELL CYTOSKELETON; MAMMALIAN-CELLS; LIPID-BILAYERS; F-ACTIN; MEMBRANE; FIELD; ELECTROPORATION; FORCE; ELECTROPERMEABILIZATION Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14771