Universität zu Köln

Diaz-Jimenez, Maria, Dorado, Jesus, Consuegra, Cesar, Pereira, Blasa, Ortiz, Isabel, Vazquez, Cristina, Isachenko, Evgenia, Isachenko, Vladimir and Hidalgo, Manuel (2019). Effect of warming temperatures on donkey sperm vitrification in 0.5 mL straws in comparison to conventional freezing. Span. J. Agric. Res., 17 (3). MADRID: SPANISH NATL INST AGRICULTURAL & FOOD RESEARCH & TECHNOLO. ISSN 2171-9292

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Abstract

Aim of study: There is little information about vitrification of sperm in large volumes (up to 0.5 mL). This study aimed to develop the vitrification technique in 0.5 mL straws in donkey sperm, evaluating the effect of three warming temperatures. Area of study: Cordoba, Spain. Material and methods: Ejaculates from five donkeys were divided in four groups: one control subjected to conventional slow freezing (C) and three vitrified in 0.5 mL straws and warmed using different protocols (W1: 37 degrees C/30s, W2: 43 degrees C/20s and W3: 70 degrees C/8s + 37 degrees C/52s). Sperm motility, kinematic parameters, plasma membrane and acrosome integrity were evaluated. Conventional freezing resulted in significantly higher values for total (42.7 +/- 19.6%), and progressive motility (30.3 +/- 16.7%), plasma membrane (49.1 +/- 10.4%) and acrosome integrity (39.6 +/- 14.5%) respect to vitrification method. Main results: Values after warming ranged between 0.2-2.8% for total motility; 0.2-2.1% for progressive motility; 5.5-20.0% for plasma membrane integrity and 14.5-29.8% for acrosome integrity in all warming protocols after sperm vitrification. However, no differences were found between W3 and C for kinematic parameters; and W3 resulted in significantly higher values for membrane integrity (20.0 +/- 11.0%) in comparison to W1 (5.5 +/- 3.6%) and W2 (9.3 +/- 8.4%). Research highlights: High warming rates seem to be better for donkey sperm vitrification in large volumes; but this methodology is still not an alternative to conventional sperm freezing.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Diaz-Jimenez, Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Dorado, Jesus UNSPECIFIED UNSPECIFIED UNSPECIFIED Consuegra, Cesar UNSPECIFIED UNSPECIFIED UNSPECIFIED Pereira, Blasa UNSPECIFIED UNSPECIFIED UNSPECIFIED Ortiz, Isabel UNSPECIFIED UNSPECIFIED UNSPECIFIED Vazquez, Cristina UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, Evgenia UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, Vladimir UNSPECIFIED UNSPECIFIED UNSPECIFIED Hidalgo, Manuel UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-160322
DOI:	10.5424/sjar/2019173-14153
Journal or Publication Title:	Span. J. Agric. Res.
Volume:	17
Number:	3
Date:	2019
Publisher:	SPANISH NATL INST AGRICULTURAL & FOOD RESEARCH & TECHNOLO
Place of Publication:	MADRID
ISSN:	2171-9292
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CRYOPROTECTANT-FREE VITRIFICATION; HUMAN SPERMATOZOA; CRYOPRESERVATION; SUPPLEMENTATION; TREHALOSE; EXTENDERS; QUALITY; PARAMETERS; FERTILITY; MOTILITY Multiple languages Agriculture, Multidisciplinary; Soil Science Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/16032