Reichel, Christian, Farmer, Letizia, Gmeiner, Guenter, Walpurgis, Katja and Thevis, Mario (2018). Detection of Sotatercept (ACE-011) in human serum by SAR-PAGE and western single blotting. Drug Test. Anal., 10 (6). S. 927 - 938. HOBOKEN: WILEY. ISSN 1942-7611
Full text not available from this repository.Abstract
A method for the detection of Sotatercept (ACE-011, ACVR2A-Fc) in human serum is presented. The method is a modification of a recently published protocol for Luspatercept (ACE-536, ACVR2B-Fc), another erythropoiesis stimulating fusion protein. Out of 27 tested antibodies against either the extracellular domain of ACVR2A or the full-length protein, only 4 antibodies bound strongly enough to Sotatercept for usage with immunoprecipitation followed by SAR-PAGE and Western single blotting. The adapted protocol allows the detection of 0.1ng/mL Sotatercept in just 50L human serum. None of the 3 commercial ACVR2-ELISAs was able to detect Sotatercept and the 2 tested surrogate proteins, even in the g/mL range. As for Luspatercept, only IPG-IEF/2D-PAGE generated discrete isoforms. Due to the long serum half-life, the SAR-PAGE method will be able to detect Sotatercept for several weeks and will be very useful in doping testing.
Item Type: | Journal Article | ||||||||||||||||||||||||
Creators: |
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URN: | urn:nbn:de:hbz:38-184715 | ||||||||||||||||||||||||
DOI: | 10.1002/dta.2346 | ||||||||||||||||||||||||
Journal or Publication Title: | Drug Test. Anal. | ||||||||||||||||||||||||
Volume: | 10 | ||||||||||||||||||||||||
Number: | 6 | ||||||||||||||||||||||||
Page Range: | S. 927 - 938 | ||||||||||||||||||||||||
Date: | 2018 | ||||||||||||||||||||||||
Publisher: | WILEY | ||||||||||||||||||||||||
Place of Publication: | HOBOKEN | ||||||||||||||||||||||||
ISSN: | 1942-7611 | ||||||||||||||||||||||||
Language: | English | ||||||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||||||
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Refereed: | Yes | ||||||||||||||||||||||||
URI: | http://kups.ub.uni-koeln.de/id/eprint/18471 |
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