Universität zu Köln

Andon, Fernando T., Mukherjee, Sourav P., Gessner, Isabel, Wortmann, Laura, Xiao, Lisong, Hultenby, Kjell, Shvedova, Anna A., Mathur, Sanjay and Fadeel, Bengt (2017). Hollow carbon spheres trigger inflammasome-dependent IL-1 beta secretion in macrophages. Carbon, 113. S. 243 - 252. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD. ISSN 1873-3891

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Abstract

It is disputed whether inflammasome activation leading to secretion of pro-inflammatory interleukin (IL)-1 beta in macrophages transpires independently of cell death or whether the two processes are linked. Here, we synthesized hollow carbon spheres (HCS) and investigated their effects on primary human monocyte-derived macrophages (HMDM); short (500 nm) non-functionalized single-walled carbon nanotubes (SWCNT) were included for comparison. HCS (250 nm) were readily taken up by HMDM and induced ROS production, but did not trigger a loss of cell viability. However, a dose- and time-dependent release of IL-1 beta was detected in lipopolysaccharide (LPS)-primed macrophages upon exposure to HCS, while SWCNT-induced secretion of IL-1 beta was less pronounced. HCS-triggered IL-1 beta secretion was cathepsin B- and caspase-1-dependent, and was accompanied by a reduction in intracellular K+. Furthermore, cytokine secretion was reduced following treatment with the antioxidant, N-acetylcysteine, and cytochalasin D, an inhibitor of actin polymerization. HCS also triggered IL-1 beta release in LPS-primed THP.1 cells, but not'in THP.1 cells with silencing of ASC, NLRP3, or caspase-1 expression, providing evidence that IL-1 beta was elicited through NLRP3 inflammasome activation. These studies shed light on the effects of HCS on primary macrophages, and show that spherical carbon-based nanoparticles are potent inflammasome activators. (C) 2016 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Andon, Fernando T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mukherjee, Sourav P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gessner, Isabel UNSPECIFIED UNSPECIFIED UNSPECIFIED Wortmann, Laura UNSPECIFIED UNSPECIFIED UNSPECIFIED Xiao, Lisong UNSPECIFIED UNSPECIFIED UNSPECIFIED Hultenby, Kjell UNSPECIFIED UNSPECIFIED UNSPECIFIED Shvedova, Anna A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mathur, Sanjay UNSPECIFIED UNSPECIFIED UNSPECIFIED Fadeel, Bengt UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-239409
DOI:	10.1016/j.carbon.2016.11.049
Journal or Publication Title:	Carbon
Volume:	113
Page Range:	S. 243 - 252
Date:	2017
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Place of Publication:	OXFORD
ISSN:	1873-3891
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Inorganic Chemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language INDUCED TOXICITY FOCUS; NLRP3 INFLAMMASOME; BIOLOGICAL INTERACTIONS; PULMONARY INFLAMMATION; OXIDE NANOPARTICLES; NALP3 INFLAMMASOME; STEM-CELLS; ACTIVATION; NANOTUBES; NANOMATERIALS Multiple languages Chemistry, Physical; Materials Science, Multidisciplinary Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/23940