Universität zu Köln

Schwarz, Jan, Bierbaum, Veronika, Merrin, Jack, Frank, Tino, Hauschild, Robert, Bollenbach, Tobias ORCID: 0000-0003-4398-476X, Tay, Savas, Sixt, Michael and Mehling, Matthias ORCID: 0000-0001-8599-1226 (2016). A microfluidic device for measuring cell migration towards substrate-bound and soluble chemokine gradients. Sci Rep, 6. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

Cellular locomotion is a central hallmark of eukaryotic life. It is governed by cell-extrinsic molecular factors, which can either emerge in the soluble phase or as immobilized, often adhesive ligands. To encode for direction, every cue must be present as a spatial or temporal gradient. Here, we developed a microfluidic chamber that allows measurement of cell migration in combined response to surface immobilized and soluble molecular gradients. As a proof of principle we study the response of dendritic cells to their major guidance cues, chemokines. The majority of data on chemokine gradient sensing is based on in vitro studies employing soluble gradients. Despite evidence suggesting that in vivo chemokines are often immobilized to sugar residues, limited information is available how cells respond to immobilized chemokines. We tracked migration of dendritic cells towards immobilized gradients of the chemokine CCL21 and varying superimposed soluble gradients of CCL19. Differential migratory patterns illustrate the potential of our setup to quantitatively study the competitive response to both types of gradients. Beyond chemokines our approach is broadly applicable to alternative systems of chemo-and haptotaxis such as cells migrating along gradients of adhesion receptor ligands vs. any soluble cue.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schwarz, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Bierbaum, Veronika UNSPECIFIED UNSPECIFIED UNSPECIFIED Merrin, Jack UNSPECIFIED UNSPECIFIED UNSPECIFIED Frank, Tino UNSPECIFIED UNSPECIFIED UNSPECIFIED Hauschild, Robert UNSPECIFIED UNSPECIFIED UNSPECIFIED Bollenbach, Tobias UNSPECIFIED orcid.org/0000-0003-4398-476X UNSPECIFIED Tay, Savas UNSPECIFIED UNSPECIFIED UNSPECIFIED Sixt, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Mehling, Matthias UNSPECIFIED orcid.org/0000-0001-8599-1226 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-255832
DOI:	10.1038/srep36440
Journal or Publication Title:	Sci Rep
Volume:	6
Date:	2016
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Physics > Institut für Biologische Physik
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MULTIPLE-SCLEROSIS; DENDRITIC CELLS; LYMPH-NODES; LIGANDS; TRAFFICKING; RECRUITMENT; CHEMOTAXIS; ACTIVATION; RECEPTOR-7; GUIDANCE Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/25583