Huang, Xin-Yuan, Chao, Dai-Yin, Koprivova, Anna, Danku, John, Wirtz, Markus ORCID: 0000-0001-7790-4022, Mueller, Steffen, Sandoval, Francisco J., Bauwe, Hermann, Roje, Sanja ORCID: 0000-0002-6302-5382, Dilkes, Brian ORCID: 0000-0003-2799-954X, Hell, Ruediger ORCID: 0000-0002-6238-4818, Kopriva, Stanislav ORCID: 0000-0002-7416-6551 and Salt, David E. (2016). Nuclear Localised MORE SULPHUR ACCUMULATION1 Epigenetically Regulates Sulphur Homeostasis in Arabidopsis thaliana. PLoS Genet., 12 (9). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1553-7404

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Abstract

Sulphur (S) is an essential element for all living organisms. The uptake, assimilation and metabolism of S in plants are well studied. However, the regulation of S homeostasis remains largely unknown. Here, we report on the identification and characterisation of the more sulphur accumulation1 (msa1-1) mutant. The MSA1 protein is localized to the nucleus and is required for both S-adenosylmethionine (SAM) production and DNA methylation. Loss of function of the nuclear localised MSA1 leads to a reduction in SAM in roots and a strong S-deficiency response even at ample S supply, causing an over-accumulation of sulphate, sulphite, cysteine and glutathione. Supplementation with SAM suppresses this high S phenotype. Furthermore, mutation of MSA1 affects genome-wide DNA methylation, including the methylation of S-deficiency responsive genes. Elevated S accumulation in msa1-1 requires the increased expression of the sulphate transporter genes SULTR1;1 and SULTR1;2 which are also differentially methylated in msa1-1. Our results suggest a novel function for MSA1 in the nucleus in regulating SAM biosynthesis and maintaining S homeostasis epigenetically via DNA methylation.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Huang, Xin-YuanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Chao, Dai-YinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Koprivova, AnnaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Danku, JohnUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wirtz, MarkusUNSPECIFIEDorcid.org/0000-0001-7790-4022UNSPECIFIED
Mueller, SteffenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sandoval, Francisco J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bauwe, HermannUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Roje, SanjaUNSPECIFIEDorcid.org/0000-0002-6302-5382UNSPECIFIED
Dilkes, BrianUNSPECIFIEDorcid.org/0000-0003-2799-954XUNSPECIFIED
Hell, RuedigerUNSPECIFIEDorcid.org/0000-0002-6238-4818UNSPECIFIED
Kopriva, StanislavUNSPECIFIEDorcid.org/0000-0002-7416-6551UNSPECIFIED
Salt, David E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-265977
DOI: 10.1371/journal.pgen.1006298
Journal or Publication Title: PLoS Genet.
Volume: 12
Number: 9
Date: 2016
Publisher: PUBLIC LIBRARY SCIENCE
Place of Publication: SAN FRANCISCO
ISSN: 1553-7404
Language: English
Faculty: Faculty of Mathematics and Natural Sciences
Divisions: Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
AFFINITY SULFATE TRANSPORTERS; ONE-CARBON METABOLISM; SERINE HYDROXYMETHYLTRANSFERASE; S-ADENOSYLMETHIONINE; FOLATE POLYGLUTAMYLATION; DNA METHYLATION; CASPARIAN STRIP; SWISS-MODEL; PROTEIN; EXPRESSIONMultiple languages
Genetics & HeredityMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/26597

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