Aldeyarbi, Hebatalla M. and Karanis, Panagiotis (2016). The fine structure of sexual stage development and sporogony of Cryptosporidium parvum in cell-free culture. Parasitology, 143 (6). S. 749 - 762. NEW YORK: CAMBRIDGE UNIV PRESS. ISSN 1469-8161
Full text not available from this repository.Abstract
The sexual stages and new oocysts development of Cryptosporidium parvum were investigated in a cell-free culture system using transmission electron microscopy (TEM). Sexual development was extremely rapid after inoculation of oocysts into the medium. The process began within 1/2-12 h and was completed with new oocyst formation 120 h post-inoculation. The macrogamonts were bounded by two membranes and had amylopectin granules and two distinct types of wall-forming bodies. The microgamonts had a large nucleus showing lobe projections and condensation of chromatin, giving rise to peripherally budding microgametes. The microgametes contained a large area of granular substance containing groups of microtubules surrounding the electron-dense nucleus. In some instances, the dividing microgamy was observed in cell-free cultures with no preceding merogonic process. Fertilization was observed with the bullet-shaped microgamete penetrating an immature macrogamont at 24 and 216 h. The new thin-and thick-walled oocysts had a large residuum with polysaccharide granules and sporogony noted inside these oocysts. Novel immature four-layer walled thick oocysts with irregular knob-like protrusions on the outer layer resembling the immature Eimeria oocysts were also observed. The present study confirms the gametogony and sporogony of C. parvum in cell-free culture and describes their ultra-structure for the first time.
Item Type: | Journal Article | ||||||||||||
Creators: |
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URN: | urn:nbn:de:hbz:38-276513 | ||||||||||||
DOI: | 10.1017/S0031182016000275 | ||||||||||||
Journal or Publication Title: | Parasitology | ||||||||||||
Volume: | 143 | ||||||||||||
Number: | 6 | ||||||||||||
Page Range: | S. 749 - 762 | ||||||||||||
Date: | 2016 | ||||||||||||
Publisher: | CAMBRIDGE UNIV PRESS | ||||||||||||
Place of Publication: | NEW YORK | ||||||||||||
ISSN: | 1469-8161 | ||||||||||||
Language: | English | ||||||||||||
Faculty: | Unspecified | ||||||||||||
Divisions: | Unspecified | ||||||||||||
Subjects: | no entry | ||||||||||||
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Refereed: | Yes | ||||||||||||
URI: | http://kups.ub.uni-koeln.de/id/eprint/27651 |
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