Hucklenbroich, Joerg, Klein, Rebecca, Neumaier, Bernd ORCID: 0000-0001-5425-3116, Graf, Rudolf, Fink, Gereon Rudolf, Schroeter, Michael and Rueger, Maria Adele (2014). Aromatic-turmerone induces neural stem cell proliferation in vitro and in vivo. Stem Cell Res. Ther., 5. LONDON: BMC. ISSN 1757-6512
Full text not available from this repository.Abstract
Introduction: Aromatic (ar-) turmerone is a major bioactive compound of the herb Curcuma longa. It has been suggested that ar-turmerone inhibits microglia activation, a property that may be useful in treating neurodegenerative disease. Furthermore, the effects of ar-turmerone on neural stem cells (NSCs) remain to be investigated. Methods: We exposed primary fetal rat NSCs to various concentrations of ar-turmerone. Thereafter, cell proliferation and differentiation potential were assessed. In vivo, naive rats were treated with a single intracerebroventricular (i.c.v.) injection of ar-turmerone. Proliferative activity of endogenous NSCs was assessed in vivo, by using noninvasive positron emission tomography (PET) imaging and the tracer [F-18]-fluoro-L-thymidine ([F-18] FLT), as well as ex vivo. Results: In vitro, ar-turmerone increased dose-dependently the number of cultured NSCs, because of an increase in NSC proliferation (P < 0.01). Proliferation data were supported by qPCR-data for Ki-67 mRNA. In vitro as well as in vivo, ar-turmerone promoted neuronal differentiation of NSCs. In vivo, after i.c.v. injection of ar-turmerone, proliferating NSCs were mobilized from the subventricular zone (SVZ) and the hippocampus of adult rats, as demonstrated by both [F-18] FLT-PET and histology (P < 0.05). Conclusions: Both in vitro and in vivo data suggest that ar-turmerone induces NSC proliferation. Ar-turmerone thus constitutes a promising candidate to support regeneration in neurologic disease.
Item Type: | Journal Article | ||||||||||||||||||||||||||||||||
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URN: | urn:nbn:de:hbz:38-428442 | ||||||||||||||||||||||||||||||||
DOI: | 10.1186/scrt500 | ||||||||||||||||||||||||||||||||
Journal or Publication Title: | Stem Cell Res. Ther. | ||||||||||||||||||||||||||||||||
Volume: | 5 | ||||||||||||||||||||||||||||||||
Date: | 2014 | ||||||||||||||||||||||||||||||||
Publisher: | BMC | ||||||||||||||||||||||||||||||||
Place of Publication: | LONDON | ||||||||||||||||||||||||||||||||
ISSN: | 1757-6512 | ||||||||||||||||||||||||||||||||
Language: | English | ||||||||||||||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||||||||||||||
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URI: | http://kups.ub.uni-koeln.de/id/eprint/42844 |
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