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Activity and electron donor preference of two denitrifying bacterial strains identified by Raman gas spectroscopy

Blohm, Annika ; Kumar, Swatantar ; Knebl, Andreas ; Herrmann, Martina ; Küsel, Kirsten ; Popp, Jürgen ; Frosch, Torsten (2024)
Activity and electron donor preference of two denitrifying bacterial strains identified by Raman gas spectroscopy.
In: Analytical and Bioanalytical Chemistry, 2022, 414 (1)
doi: 10.26083/tuprints-00023436
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Item Type: Article
Type of entry: Secondary publication
Title: Activity and electron donor preference of two denitrifying bacterial strains identified by Raman gas spectroscopy
Language: English
Date: 18 March 2024
Place of Publication: Darmstadt
Year of primary publication: January 2022
Place of primary publication: Berlin ; Heidelberg
Publisher: Springer
Journal or Publication Title: Analytical and Bioanalytical Chemistry
Volume of the journal: 414
Issue Number: 1
DOI: 10.26083/tuprints-00023436
Corresponding Links:
Origin: Secondary publication DeepGreen
Abstract:

Human activities have greatly increased the input of reactive nitrogen species into the environment and disturbed the balance of the global N cycle. This imbalance may be offset by bacterial denitrification, an important process in maintaining the ecological balance of nitrogen. However, our understanding of the activity of mixotrophic denitrifying bacteria is not complete, as most research has focused on heterotrophic denitrification. The aim of this study was to investigate substrate preferences for two mixotrophic denitrifying bacterial strains, Acidovorax delafieldii and Hydrogenophaga taeniospiralis, under heterotrophic, autotrophic or mixotrophic conditions. This complex analysis was achieved by simultaneous identification and quantification of H₂, O₂, CO₂, ¹⁴N₂, ¹⁵N₂ and ¹⁵N₂O in course of the denitrification process with help of cavity-enhanced Raman spectroscopic (CERS) multi-gas analysis. To disentangle electron donor preferences for both bacterial strains, microcosm-based incubation experiments under varying substrate conditions were conducted. We found that Acidovorax delafieldii preferentially performed heterotrophic denitrification in the mixotrophic sub-experiments, while Hydrogenophaga taeniospiralis preferred autotrophic denitrification in the mixotrophic incubation. These observations were supported by stoichiometric calculations. The results demonstrate the prowess of advanced Raman multi-gas analysis to study substrate use and electron donor preferences in denitrification, based on the comprehensive quantification of complex microbial gas exchange processes.

Uncontrolled Keywords: Cavity-enhanced Raman spectroscopy, Denitrification, Multi-gas analysis, Nitrogen cycle
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-234368
Classification DDC: 500 Science and mathematics > 540 Chemistry
500 Science and mathematics > 570 Life sciences, biology
600 Technology, medicine, applied sciences > 620 Engineering and machine engineering
Divisions: 18 Department of Electrical Engineering and Information Technology > Biophotonik-Medizintechnik
Date Deposited: 18 Mar 2024 13:34
Last Modified: 30 Apr 2024 09:35
SWORD Depositor: Deep Green
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/23436
PPN: 517365537
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